Generation of CHOPi014-A from healthy adult peripheral blood mononuclear cells

Abstract

CHOPWT15 is a control male induced pluripotent stem cell (iPSC) line that can be used to model genetic variants by creating an allelic series of isogenic lines using genome editing technologies (Hockemeyer and Jaenisch, 2016, Maguire et al., 2022, Pavani et al., 2022). An allelic series of iPSC lines provides the means to perform genotype and phenotype analyses of pathological variants without the confounding effects of genetic background. Peripheral blood mononuclear cells (PBMCs), obtained from a healthy adult male, were reprogrammed using Sendai virus creating an iPSC line that has undetectable copy number variations (CNVs, resolution >= 100 kb) and differentiates to all three germlayers.