Targeted next-generation sequencing (tNGS): An upcoming application for pathogen identification in clinical diagnosis

IF 4 3区医学 Q1 INFECTIOUS DISEASES

Journal of Infection and Public Health Pub Date : 2025-08-21 DOI:10.1016/j.jiph.2025.102936

Ao Liang , Xiaoxue Wu , Yaqi Zhu , Lixian Pan , Anbang Wang , Chunchen Wu , Jianbo Xia

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引用次数: 0

Abstract

Rapid and efficient detection of pathogens is a goal for clinical laboratories. Traditional methods such as post-culture identification, smear microscopy, antigen-antibody detection, and polymerase chain reaction cannot perform hundreds of high-throughput tests simultaneously. With the development of genetic engineering, next-generation sequencing technology has gradually been introduced into clinical testing. The new technology of targeted next-generation sequencing (tNGS) has the advantages of high sensitivity, high efficiency, and relatively low cost. This review of articles on tNGS published in the past two decades summarizes tNGS workflow and clinical applications, compares it with traditional culture and metagenomic next-generation sequencing (mNGS), and highlights its role in detecting multiple pathogens in mixed infections and drug-resistance genes. It also elaborates on the latest guidelines and the quality control process. By highlighting the practical clinical use of tNGS, it offers insights for optimizing the detection of multiple pathogens in polymicrobial infections to enhance diagnostic accuracy and efficiency.

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靶向新一代测序（tNGS）：病原体鉴定在临床诊断中的应用前景

快速有效地检测病原体是临床实验室的目标。传统的方法，如培养后鉴定、涂片显微镜、抗原抗体检测和聚合酶链反应不能同时进行数百种高通量检测。随着基因工程技术的发展，新一代测序技术逐渐被引入到临床检测中。新一代靶向测序技术具有灵敏度高、效率高、成本相对较低等优点。本文综述了近20年来有关tNGS的研究成果，总结了tNGS的工作流程和临床应用，并将其与传统培养和新一代宏基因组测序（mNGS）进行了比较，重点介绍了tNGS在混合感染和耐药基因中检测多种病原体的作用。它还详细阐述了最新的指导方针和质量控制过程。通过强调tNGS的实际临床应用，为优化多微生物感染中多种病原体的检测提供了见解，以提高诊断的准确性和效率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Infection and Public Health PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH -INFECTIOUS DISEASES

CiteScore

13.10

自引率

1.50%

发文量

203

审稿时长

96 days

期刊介绍： The Journal of Infection and Public Health, first official journal of the Saudi Arabian Ministry of National Guard Health Affairs, King Saud Bin Abdulaziz University for Health Sciences and the Saudi Association for Public Health, aims to be the foremost scientific, peer-reviewed journal encompassing infection prevention and control, microbiology, infectious diseases, public health and the application of healthcare epidemiology to the evaluation of health outcomes. The point of view of the journal is that infection and public health are closely intertwined and that advances in one area will have positive consequences on the other. The journal will be useful to all health professionals who are partners in the management of patients with communicable diseases, keeping them up to date. The journal is proud to have an international and diverse editorial board that will assist and facilitate the publication of articles that reflect a global view on infection control and public health, as well as emphasizing our focus on supporting the needs of public health practitioners. It is our aim to improve healthcare by reducing risk of infection and related adverse outcomes by critical review, selection, and dissemination of new and relevant information in the field of infection control, public health and infectious diseases in all healthcare settings and the community.