Recruitment of Aβ into α-Synuclein Condensates Catalyzes Primary Nucleation of α-Synuclein Aggregation

IF 10.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Owen M. Morris, Alexander Röntgen, Zenon Toprakcioglu*, Mariana Cali, Samuel Dada and Michele Vendruscolo*, 
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引用次数: 0

Abstract

The aggregation of amyloid-β (Aβ) and α-synuclein (αSyn) is linked to Alzheimer’s and Parkinson’s diseases, with growing evidence suggesting possible interactions between Aβ and αSyn in the pathology of these neurodegenerative conditions. In this context, the recent observation that protein aggregation into amyloid fibrils may take place within liquid condensates generated through liquid–liquid phase separation prompts the question of how amyloidogenic proteins interact with each other, and more specifically whether Aβ can influence the overall phase behavior of αSyn or vice versa. To address this question, we investigated the interplay between Aβ40, the most abundant form of Aβ, with αSyn. We found that monomeric Aβ40 is sequestered into αSyn condensates, where it enhances heterogeneous primary nucleation, and accelerates the aggregation of αSyn within the liquid condensates. Using a chemical kinetics framework, we further showed that this liquid-to-solid transition is not significantly affected by adding preformed Aβ40 fibrillar seeds, further indicating that monomeric Aβ40 specifically enhances the primary nucleation of αSyn within the condensed phase. These findings identify some of the key mechanistic processes underlying amyloid aggregation within liquid condensates, prompting further investigations into the possible role of Aβ and αSyn cocondensation interactions in the onset and progression of neurodegenerative disorders.

This study reports the effect of amyloid-β (Aβ) on the phase transitions of α-synuclein (αSyn), showing that Aβ40 is recruited into αSyn condensates where it accelerates αSyn amyloid aggregation.

α-Synuclein缩合物募集α β催化α-Synuclein聚集的初成核
淀粉样蛋白-β (Aβ)和α-突触核蛋白(αSyn)的聚集与阿尔茨海默病和帕金森病有关,越来越多的证据表明,Aβ和αSyn可能在这些神经退行性疾病的病理中相互作用。在这种背景下,最近的观察表明,通过液液相分离产生的液体凝聚物中可能发生蛋白质聚集成淀粉样原纤维,这引发了淀粉样蛋白如何相互作用的问题,更具体地说,Aβ是否会影响αSyn的整体相行为,反之亦然。为了解决这个问题,我们研究了Aβ最丰富的形式Aβ40与αSyn之间的相互作用。研究发现,单体Aβ40在αSyn凝聚体中固存,增强了αSyn的非均相初生成核,加速了αSyn在液相凝聚体中的聚集。利用化学动力学框架,我们进一步发现,添加预成型的a β40纤维种子对这种液固转变没有显著影响,进一步表明单体a β40特异性地增强了凝聚相中αSyn的初成核。这些发现确定了液体凝聚物中淀粉样蛋白聚集的一些关键机制过程,促进了对Aβ和αSyn凝聚相互作用在神经退行性疾病发生和进展中的可能作用的进一步研究。本研究报道了淀粉样蛋白-β (Aβ)对α-突触核蛋白(αSyn)相变的影响,表明Aβ40被招募到αSyn凝聚体中,并加速αSyn淀粉样蛋白的聚集。
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来源期刊
ACS Central Science
ACS Central Science Chemical Engineering-General Chemical Engineering
CiteScore
25.50
自引率
0.50%
发文量
194
审稿时长
10 weeks
期刊介绍: ACS Central Science publishes significant primary reports on research in chemistry and allied fields where chemical approaches are pivotal. As the first fully open-access journal by the American Chemical Society, it covers compelling and important contributions to the broad chemistry and scientific community. "Central science," a term popularized nearly 40 years ago, emphasizes chemistry's central role in connecting physical and life sciences, and fundamental sciences with applied disciplines like medicine and engineering. The journal focuses on exceptional quality articles, addressing advances in fundamental chemistry and interdisciplinary research.
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