Isolation and Genetic Characterization of Three Palyam Serogroup Orbiviruses From Culicoides Spp. and Associated Infections in Cattle in Yunnan, China

IF 3 2区农林科学 Q2 INFECTIOUS DISEASES

Transboundary and Emerging Diseases Pub Date : 2025-08-25 DOI:10.1155/tbed/6615175

Zhenxing Yang, Yuwen He, Susheng Li, Jinxin Meng, Nan Li, Jinglin Wang

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Abstract

Palyam serogroup virus (PALV) is a potential pathogen prevalent in Asia, Australia, and Africa that may cause abortion and teratogenesis in ruminants, especially cattle. In this study, we isolated three PALV strains (MY_C17, ML_C40, and SZ_C06) from Culicoides spp. in Yunnan, China, and obtained their complete genome sequences via next-generation sequencing. The phylogenetic analysis of Seg-5, Seg-7, and Seg-9 indicated that PALVs can be grouped based on their geographical origins: Asia, Australia, and Africa. The three isolates in this study belong to the Asia group. However, the Central African Republic strain (AR_B_2032) and the India strain (DVTD) form a separate branch, provisionally named the CI group. In the VP2 phylogenetic tree, all PALV strains can be grouped into six significant branches, designated as A through F. The three isolates are clustered in Groups A, E, and F and are shown to belong to the D’Aguilar virus (DAGV), Chuzan virus (CHUV), and Bunyip Creek virus (BCV) serotypes, respectively. Seroepidemiological surveys were conducted on local cattle using neutralization tests, which revealed seroprevalence rates of neutralizing antibodies against BVC, DAGV, and CHUV in Yunnan cattle serum of 3.4% (12/350), 6.9% (24/350), and 16.9% (59/350), respectively. This marks the first-ever isolation of CHUV, specifically BCV and DAGV, from vector Culicoides spp. in Southwest China. It was also proved that in this region, at least three serotypes of PALV circulated among Culicoides spp. in nature and infected domestic cattle.

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云南牛库蠓属三种Palyam血清型病毒的分离与遗传特征

Palyam血清群病毒（PALV）是一种流行于亚洲、澳大利亚和非洲的潜在病原体，可引起反刍动物，特别是牛的流产和致畸。本研究从云南库蠓中分离到3株PALV (MY_C17、ML_C40和SZ_C06)，并通过下一代测序获得了它们的全基因组序列。Seg-5、Seg-7和Seg-9的系统发育分析表明，palv可以根据其地理起源进行分组：亚洲、澳大利亚和非洲。本研究的三个分离株属于亚洲组。然而，中非共和国毒株（AR_B_2032）和印度毒株（DVTD）形成了一个单独的分支，暂时命名为CI群。在VP2系统发育树中，所有PALV毒株可分为6个重要分支，分别为A ~ F。3个分离株聚集在A、E和F组，分别属于D’aguilar病毒（DAGV）、Chuzan病毒（CHUV）和Bunyip Creek病毒（BCV）血清型。采用中和试验对当地牛进行血清流行病学调查，结果显示云南牛血清中BVC、DAGV和CHUV的中和抗体阳性率分别为3.4%（12/350）、6.9%（24/350）和16.9%（59/350）。这标志着中国西南地区首次从库蠓媒介中分离出CHUV，特别是BCV和DAGV。该地区至少有三种血清型的PALV在自然界库蠓和感染家畜之间传播。

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来源期刊

Transboundary and Emerging Diseases 农林科学-传染病学

CiteScore

8.90

自引率

9.30%

发文量

350

审稿时长

1 months

期刊介绍： Transboundary and Emerging Diseases brings together in one place the latest research on infectious diseases considered to hold the greatest economic threat to animals and humans worldwide. The journal provides a venue for global research on their diagnosis, prevention and management, and for papers on public health, pathogenesis, epidemiology, statistical modeling, diagnostics, biosecurity issues, genomics, vaccine development and rapid communication of new outbreaks. Papers should include timely research approaches using state-of-the-art technologies. The editors encourage papers adopting a science-based approach on socio-economic and environmental factors influencing the management of the bio-security threat posed by these diseases, including risk analysis and disease spread modeling. Preference will be given to communications focusing on novel science-based approaches to controlling transboundary and emerging diseases. The following topics are generally considered out-of-scope, but decisions are made on a case-by-case basis (for example, studies on cryptic wildlife populations, and those on potential species extinctions): Pathogen discovery: a common pathogen newly recognised in a specific country, or a new pathogen or genetic sequence for which there is little context about — or insights regarding — its emergence or spread. Prevalence estimation surveys and risk factor studies based on survey (rather than longitudinal) methodology, except when such studies are unique. Surveys of knowledge, attitudes and practices are within scope. Diagnostic test development if not accompanied by robust sensitivity and specificity estimation from field studies. Studies focused only on laboratory methods in which relevance to disease emergence and spread is not obvious or can not be inferred (“pure research” type studies). Narrative literature reviews which do not generate new knowledge. Systematic and scoping reviews, and meta-analyses are within scope.