Functional activities of Lacticaseibacillus casei in culture of Oplegnathus punctatus

IF 2.4 3区农林科学 Q2 FISHERIES

Aquaculture International Pub Date : 2025-08-26 DOI:10.1007/s10499-025-02189-0

Yin Han, Duoxiang Zhao, Da Liang, Yuanyuan Sun, Wenjie Wang, Jiansong Chu, Zizhong Qi

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Abstract

This study investigated the survival dynamics of Lacticaseibacillus casei YB-38 in nutrient-rich seawater and its inhibitory effect on the pathogenic bacterium Vibrio harveyi YL-10. L. casei YB-38 at 10⁸ CFU per gram of feed was supplemented with feed in the cultivation of Oplegnathus punctatus (initial average weight = 5.30 ± 0.20 g), focusing on the growth characteristics for 14 days. Flow cytometry and genomics analysis were utilized to investigate the effects of L. casei YB-38 on the intestinal microbiota activity and immune function of O. punctatus. The results indicated that L. casei YB-38 and V. harveyi YL-10 demonstrate enhanced growth under simulated enriched conditions. V. harveyi YL-10 exhibited better growth when attaching feed particles in seawater, irrespective of feed addition. L. casei YB-38 at a concentration of 10⁷ CFU/mL inhibited V. harveyi YL-10, which was initially present at 10³ CFU/mL, and the inhibition lasted for 8 h. The addition of L. casei to the feed significantly enhanced the growth of O. punctatus, and modulated the gut microbiota by reducing the relative abundance of the genus Vibrio and increasing that of Lactobacillus. O. punctatus fed with L. casei YB-38 exhibited smaller fluctuations in intestinal microbiota compared to the control group, indicating the stabilizing effect of L. casei YB-38 on the intestinal microbiota. Furthermore, the inclusion of L. casei YB-38 in the feed potentially bolstered the growth metabolism of O. punctatus, including pathways related to protein digestion and absorption, and antigen processing.

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马尾鱼培养中干酪乳杆菌的功能活性研究

本研究研究了干酪乳杆菌YB-38在富营养化海水中的生存动态及其对致病菌哈维弧菌YL-10的抑制作用。以108 CFU / g饲料水平的酪氨酸乳杆菌YB-38添加饲料，对初始平均体重= 5.30±0.20 g的马尾鱼进行培养，观察其生长特性，为期14 d。采用流式细胞术和基因组学方法研究了干酪乳杆菌YB-38对马尾蚴肠道菌群活性和免疫功能的影响。结果表明，在模拟富集条件下，干酪乳杆菌（L. casei） YB-38和哈维芽孢杆菌（V. harveyi） YL-10的生长明显增强。无论饲料添加量如何，harveyi YL-10在海水中附着饲料颗粒时均表现出较好的生长。10⁷CFU/mL浓度的干酪乳杆菌YB-38抑制了最初存在于103 CFU/mL浓度的V. harveyi YL-10，抑制持续了8小时。饲料中添加干酪乳杆菌显著促进了O. punctatus的生长，并通过降低弧菌属的相对丰度和增加乳酸杆菌的相对丰度来调节肠道微生物群。与对照组相比，饲喂干酪乳杆菌YB-38的马尾鱼肠道菌群波动较小，说明干酪乳杆菌YB-38对马尾鱼肠道菌群具有稳定作用。此外，在饲料中加入酪蛋白酪蛋白YB-38可能会促进斑点O.的生长代谢，包括与蛋白质消化和吸收以及抗原加工相关的途径。

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来源期刊

Aquaculture International 农林科学-渔业

CiteScore

5.10

自引率

6.90%

发文量

204

审稿时长

1.0 months

期刊介绍： Aquaculture International is an international journal publishing original research papers, short communications, technical notes and review papers on all aspects of aquaculture. The Journal covers topics such as the biology, physiology, pathology and genetics of cultured fish, crustaceans, molluscs and plants, especially new species; water quality of supply systems, fluctuations in water quality within farms and the environmental impacts of aquacultural operations; nutrition, feeding and stocking practices, especially as they affect the health and growth rates of cultured species; sustainable production techniques; bioengineering studies on the design and management of offshore and land-based systems; the improvement of quality and marketing of farmed products; sociological and societal impacts of aquaculture, and more. This is the official Journal of the European Aquaculture Society.