Application of ABA and kinetin on the aquatic carnivorous plant Aldrovanda vesiculosa affects the morphology and phytohormone profiles in shoot apices

IF 2.6 4区生物学 Q2 MARINE & FRESHWATER BIOLOGY

Aquatic Botany Pub Date : 2025-08-22 DOI:10.1016/j.aquabot.2025.103942

Lubomír Adamec , Lenka Plačková , Dardan Klos , Karel Doležal

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Abstract

Aldrovanda vesiculosa is a rootless aquatic carnivorous plant forming turions (winter buds) as specific overwintering organs containing high abscisic acid (ABA) content. The high ABA content has the key role in regulating turion formation and maintaining turion dormancy. Here, we compared hormonal profiles (cytokinins, auxins, ABA) in shoot apices in A. vesiculosa grown in mini aquaria in a greenhouse at the end of the summer season after a 2-day priming with ABA (2 µM) and ABA together with an artificial cytokinin kinetin (KIN, 20 µM), to find out their effects and interactions on turion formation and morphology. During the next 11 days (without added hormones), the ABA application gently boosted the turion development but the rapid apical shoot growth was not influenced. The shoot apices were narrower and more condensed. The ABA+KIN application almost ceased the apical growth and retarded the turion development. Shoot apices were robust and wide. Turion development may thus interfere with apical shoot growth. The application of ABA alone did not significantly change the contents of cytokinins, auxins or ABA in shoot apices, but +ABA+KIN significantly decreased the total and active endogenous cytokinins and increased the ABA content threefold compared to the control. Thus, both added hormones affected the developing turions in a similar way which is known and expected in dormant organs of terrestrial plants. These results suggest that the coordination between ABA and cytokinins might be crucial for hormonal regulation of the onset of turion dormancy and turion development in aquatic plants.

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ABA和动素对水生食肉植物水仙花茎尖形态和植物激素分布的影响

水草是一种无根的水生食肉植物，其特有的越冬器官为冬芽，具有较高的ABA含量。高ABA含量在调节鱼鳞形成和维持鱼鳞休眠中起着关键作用。在此，我们比较了夏末在温室迷你水族箱中生长的水仙花茎尖的激素（细胞分裂素、生长素、ABA），并在ABA（2 µM）和ABA与人工细胞分裂素（KIN, 20 µM）的作用下，研究了它们对水仙花形成和形态的影响和相互作用。在随后的11 d内（未添加激素），外源ABA的施用对斑马鱼的生长有轻微的促进作用，但对斑马鱼根尖的快速生长没有影响。茎尖更窄，更紧凑。ABA+KIN的施用几乎使其顶端生长停止，扭转发育迟缓。茎尖粗壮而宽。因此，Turion的发育可能会干扰顶芽的生长。单独施用ABA对茎尖细胞分裂素、生长素和ABA含量没有显著影响，但与对照相比，+ABA+KIN显著降低了总内源细胞分裂素和活性细胞分裂素，使ABA含量增加了3倍。因此，这两种添加的激素以类似的方式影响发育中的旋转体，这在陆地植物的休眠器官中是已知和预期的。这些结果表明，ABA和细胞分裂素之间的协同作用可能是水生植物中激素调控鱼鞘休眠和发育的关键。

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来源期刊

Aquatic Botany 生物-海洋与淡水生物学

CiteScore

3.80

自引率

5.60%

发文量

审稿时长

6 months

期刊介绍： Aquatic Botany offers a platform for papers relevant to a broad international readership on fundamental and applied aspects of marine and freshwater macroscopic plants in a context of ecology or environmental biology. This includes molecular, biochemical and physiological aspects of macroscopic aquatic plants as well as the classification, structure, function, dynamics and ecological interactions in plant-dominated aquatic communities and ecosystems. It is an outlet for papers dealing with research on the consequences of disturbance and stressors (e.g. environmental fluctuations and climate change, pollution, grazing and pathogens), use and management of aquatic plants (plant production and decomposition, commercial harvest, plant control) and the conservation of aquatic plant communities (breeding, transplantation and restoration). Specialized publications on certain rare taxa or papers on aquatic macroscopic plants from under-represented regions in the world can also find their place, subject to editor evaluation. Studies on fungi or microalgae will remain outside the scope of Aquatic Botany.