Inactivation of BSL-2 and BSL-3 human pathogens using FATHHOME’s Trinion Disinfector: A rapid and eco-friendly ozone-based dry disinfection approach

IF 3 Q1 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
Kabita Adhikari , Elizabeth Zhou , Majid Khan , Shubhasish Goswami , Amir Khazaieli , Blake A. Simmons , Deepika Awasthi , Subhash C. Verma
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引用次数: 0

Abstract

The role of personal protective equipment (PPE) in protecting against exposure to infectious agents and toxic chemicals is well-established. However, the global surge in PPE demand during the pandemic exposed challenges, including shortages and environmental impacts from disposable waste. Developing effective, scalable, and sustainable decontamination methods for the reuse of PPE is essential. Ozone has emerged as a promising, eco-friendly disinfectant due to its strong oxidative properties, rapid action, and residue-free breakdown into oxygen. This study evaluates the effectiveness of the FATHHOME Trinion Disinfector, an innovative ozone-based dry sterilization device, for inactivating pathogens on PPE materials, such as not resistant to oil 95 (N95) masks and face shields. The device’s bactericidal performance was tested against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella typhimurium, Enterococcus durans, Enterococcus faecalis, and Saccharomyces cerevisiae, achieving a 1- to 2-log reduction in these bacterial and fungal pathogens. A 30-minute ozone exposure cycle was found to attain maximum sterilization efficiency. We also demonstrated the disinfector’s efficacy against viral pathogens, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), adeno-associated virus (AAV), herpes simplex virus type 1 (HSV-1), and hepatitis B virus (HBV) on PPE surfaces. SARS-CoV-2 contamination on face shields and N95 masks decreased by 99.9 %, and AAV infectivity was nearly eliminated. Similar reductions were observed for HSV-1 and HBV. Overall, the findings confirm that ozone-based disinfection offers a rapid, scalable, and sustainable method for decontaminating PPE. These results support the establishment of standardized ozone disinfection protocols to enhance infection control, address PPE shortages, and minimize environmental waste.
使用FATHHOME的Trinion消毒器灭活BSL-2和BSL-3人类病原体:一种快速和环保的臭氧干消毒方法
个人防护装备(PPE)在防止接触传染原和有毒化学品方面的作用是公认的。然而,大流行期间全球个人防护装备需求激增带来了挑战,包括短缺和一次性废物对环境的影响。制定有效、可扩展和可持续的个人防护装备净化方法至关重要。臭氧因其强大的氧化特性、快速的作用和无残留物分解成氧气而成为一种有前途的环保消毒剂。本研究评估了FATHHOME Trinion消菌器的有效性,该消菌器是一种创新的臭氧干式灭菌装置,用于灭活PPE材料上的病原体,如不耐油95 (N95)口罩和面罩。该装置对大肠杆菌、铜绿假单胞菌、金黄色葡萄球菌、鼠伤寒沙门氏菌、durans肠球菌、粪肠球菌和酿酒酵母的杀菌性能进行了测试,这些细菌和真菌病原体的杀灭率降低了1- 2倍。发现30分钟的臭氧暴露周期可达到最大的灭菌效率。我们还证明了消毒剂对PPE表面的病毒性病原体,严重急性呼吸综合征冠状病毒2 (SARS-CoV-2),腺相关病毒(AAV),单纯疱疹病毒1型(HSV-1)和乙型肝炎病毒(HBV)的有效性。口罩和N95口罩上的SARS-CoV-2污染下降99.9%,基本消除了AAV感染。在HSV-1和HBV中观察到类似的减少。总体而言,研究结果证实,基于臭氧的消毒提供了一种快速、可扩展和可持续的个人防护装备去污方法。这些结果支持建立标准化的臭氧消毒方案,以加强感染控制,解决个人防护装备短缺问题,并尽量减少环境浪费。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biosafety and Health
Biosafety and Health Medicine-Infectious Diseases
CiteScore
7.60
自引率
0.00%
发文量
116
审稿时长
66 days
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