Source control of acid mine drainage production by sulfate reducing bacteria community: Effect and mechanism

IF 5 2区工程技术 Q1 ENGINEERING, CHEMICAL

Minerals Engineering Pub Date : 2025-08-26 DOI:10.1016/j.mineng.2025.109746

Qi Jin, Tianyu Zhi, Hai Lin

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引用次数: 0

Abstract

The application of the sulfate-reducing bacteria community (SRBC) for acid mine drainage (AMD) bioremediation has attracted growing interest. However, its potential for in situ suppression of acid generation from mine waste rocks remains unexplored. This study investigates the oxygen-inhibition and acid-control capabilities of SRBC using a low-cost carbon source (extract LP and alkali-pretreated peanut shells) through a series of static experiments. Results demonstrate that SRBC effectively suppresses acid production in waste rocks, achieving a 92 % inhibition rate for pyrite oxidation and maintaining pH above 6 for over 35 days. Even in the presence of Acidithiobacillus ferrooxidans (At.f), SRBC keep the pH above 6 and the oxidation–reduction potential (ORP) below −150 mV, confirming its robust inhibitory effect. Microbial community analysis revealed enhanced richness and diversity following pyrite exposure. X-ray diffraction (XRD) and scanning electron microscopy (SEM) analyses further indicate that SRBC mitigates acid generation by reducing SO₄²⁻ to S²⁻, which subsequently precipitates with Fe ions as metal sulfides. This study highlights SRBC’s cost-effective and sustainable potential for in situ waste rock stabilization, providing a foundation for its practical implementation in mine remediation.

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硫酸盐还原菌群落对酸性矿井水生产的源头控制：效果与机理

硫酸盐还原菌群落（SRBC）在酸性矿井污水（AMD）生物修复中的应用日益引起人们的关注。然而，其在矿山废石就地抑酸方面的潜力尚未探明。本研究通过一系列静态实验研究了低成本碳源（提取LP和碱预处理花生壳）对SRBC的抑氧抑酸能力。结果表明，SRBC有效抑制废石中的产酸，对黄铁矿氧化的抑制率达到92%，并将pH值维持在6以上35天以上。即使在酸性氧化亚铁硫杆菌(At。f)， SRBC保持pH在6以上，氧化还原电位（ORP）在−150 mV以下，证实了其强大的抑制作用。微生物群落分析表明，黄铁矿暴露后微生物群落丰富度和多样性增强。x射线衍射（XRD）和扫描电镜（SEM）分析进一步表明，SRBC通过将SO42−还原为S2−来减轻酸的产生，S2−随后与Fe离子作为金属硫化物析出。该研究突出了SRBC在废石原位稳定中的成本效益和可持续潜力，为其在矿山修复中的实际应用提供了基础。

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来源期刊

Minerals Engineering 工程技术-工程：化工

CiteScore

8.70

自引率

18.80%

发文量

519

审稿时长

81 days

期刊介绍： The purpose of the journal is to provide for the rapid publication of topical papers featuring the latest developments in the allied fields of mineral processing and extractive metallurgy. Its wide ranging coverage of research and practical (operating) topics includes physical separation methods, such as comminution, flotation concentration and dewatering, chemical methods such as bio-, hydro-, and electro-metallurgy, analytical techniques, process control, simulation and instrumentation, and mineralogical aspects of processing. Environmental issues, particularly those pertaining to sustainable development, will also be strongly covered.