Smartphone-assisted colorimetric detection of phosphate ion based on CeFe bi-metal-organic framework nanozyme

IF 4.7 3区化学 Q2 CHEMISTRY, PHYSICAL

Journal of Photochemistry and Photobiology A-chemistry Pub Date : 2025-08-21 DOI:10.1016/j.jphotochem.2025.116713

Feng Zhao, Zhenhui Yue, Zhen Lei

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引用次数: 0

Abstract

Phosphate ion (Pi) is a critical indicator for eutrophication of environmental water, and it is important to develop sensitive and accurate method for Pi detection. In this work, a signal-off colorimetric sensor for Pi detection was developed based on CeFe bi-metal-organic framework nanozyme (MIL-88A_Ce/Fe NPs). The MIL-88A_Ce/Fe NPs were simply synthesized at room temperature, and exhibited peroxidase (POD)-like, oxidase-like, superoxide dismutase-like and phosphatase-like activity. The synergistic effect of Ce³⁺/Ce⁴⁺ and Fe²⁺/Fe³⁺ redox pairs endowed MIL-88A_Ce/Fe NPs with higher POD-like activity. The specific activity was calculated to be 2.246 U mg⁻¹. The catalytic mechanism was ascribed to nanozyme-promoted generation of hydroxyl radical. The POD-mimicking activity followed Michaelis-Menten kinetics. The Michaelis constant (K_m) and maximum velocity (V_max) were 0.2788 mM and 10.88 × 10⁻⁸ M s⁻¹ for TMB, while 0.4598 mM and 12.07 × 10⁻⁸ M s⁻¹ for H₂O₂. MIL-88A_Ce/Fe NPs retained 85 % of catalytic activity after stored for 65 days, showing high stability. The Ce species in MIL-88A_Ce/Fe NPs served as the recognition sites for Pi, and the interaction with Pi inhibited the POD-like activity of MIL-88A_Ce/Fe NPs. Thus, a signal-off colorimetric assay for Pi detection was proposed. The method exhibited low detection limit, short assay time, good selectivity and strong anti-interference ability. By proceeding the sensing system on test paper, a smartphone-assisted portable colorimetric platform was constructed to achieve on-site detection. Moreover, the method was used to determine Pi in real water samples, good recoveries (99.27 %-102.86 %) and low relative standard deviations (≤4.64 %) were obtained, showing great potential for environment monitoring.

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基于CeFe双金属-有机骨架纳米酶的智能手机辅助比色法检测磷酸离子

磷酸盐离子（Pi）是环境水体富营养化的重要指标，建立灵敏、准确的Pi检测方法具有重要意义。本文基于CeFe双金属-有机框架纳米酶（MIL-88ACe/Fe NPs），研制了一种用于Pi检测的信号关闭比色传感器。MIL-88ACe/Fe NPs在室温下简单合成，具有过氧化物酶（POD）样、氧化酶样、超氧化物歧化酶样和磷酸酶样活性。Ce3+/Ce4+和Fe2+/Fe3+氧化还原对的协同作用使MIL-88ACe/Fe NPs具有较高的pod样活性。比活性为2.246 U mg−1。纳米酶促进羟基自由基的生成是催化机理。pod模拟活动遵循Michaelis-Menten动力学。TMB的Michaelis常数（Km）和最大流速（Vmax）分别为0.2788 mM和10.88 × 10−8 M s−1，H2O2的Michaelis常数（Km）和最大流速（Vmax）分别为0.4598 mM和12.07 × 10−8 M s−1。MIL-88ACe/Fe NPs保存65天后仍保持85%的催化活性，具有较高的稳定性。MIL-88ACe/Fe NPs中的Ce是Pi的识别位点，与Pi的相互作用抑制了MIL-88ACe/Fe NPs的pod样活性。因此，提出了一种用于Pi检测的信号关闭比色法。该方法检出限低，检测时间短，选择性好，抗干扰能力强。通过在试卷上进行传感系统，构建智能手机辅助便携式比色平台，实现现场检测。将该方法应用于实际水样中，回收率（99.27% ~ 102.86%）好，相对标准偏差（≤4.64%）低，具有良好的环境监测潜力。

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来源期刊

Journal of Photochemistry and Photobiology A-chemistry 化学-物理化学

CiteScore

7.90

自引率

7.00%

发文量

580

审稿时长

48 days

期刊介绍： JPPA publishes the results of fundamental studies on all aspects of chemical phenomena induced by interactions between light and molecules/matter of all kinds. All systems capable of being described at the molecular or integrated multimolecular level are appropriate for the journal. This includes all molecular chemical species as well as biomolecular, supramolecular, polymer and other macromolecular systems, as well as solid state photochemistry. In addition, the journal publishes studies of semiconductor and other photoactive organic and inorganic materials, photocatalysis (organic, inorganic, supramolecular and superconductor). The scope includes condensed and gas phase photochemistry, as well as synchrotron radiation chemistry. A broad range of processes and techniques in photochemistry are covered such as light induced energy, electron and proton transfer; nonlinear photochemical behavior; mechanistic investigation of photochemical reactions and identification of the products of photochemical reactions; quantum yield determinations and measurements of rate constants for primary and secondary photochemical processes; steady-state and time-resolved emission, ultrafast spectroscopic methods, single molecule spectroscopy, time resolved X-ray diffraction, luminescence microscopy, and scattering spectroscopy applied to photochemistry. Papers in emerging and applied areas such as luminescent sensors, electroluminescence, solar energy conversion, atmospheric photochemistry, environmental remediation, and related photocatalytic chemistry are also welcome.