Cytoplasmic and nuclear Survivin expression in healthy gastric mucosae of equids: A comparative study between horses, donkeys, and mules

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science Pub Date : 2025-08-18 DOI:10.1016/j.jevs.2025.105678

A.L. Medina-Bolívar , J.D. Muñoz-Duque , J.R. Martínez-Aranzales

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引用次数: 0

Abstract

Background

Information regarding Survivin protein expression in the gastric mucosa of equids is scarce. This protein has been associated with functions related to modulating apoptosis and promoting mitosis in epithelial cells and is considered part of gastric cytoprotection mechanisms in humans and mice, maintaining mucosal integrity and regulating cellular renewal.

Aims/objectives

This study aimed to determine the expression of cytoplasmic and nuclear Survivin in gastric mucosae of equids (horses, donkeys, and mules).

Methods

A total of 30 healthy stomachs, 10 from each species, were evaluated using histology and immunohistochemistry techniques. Survivin protein was detected with antibody EP119 and with the polymer method.

Results

Immunohistochemistry revealed survivin expression in the gastric mucosa of equids. In horses and mules, the survivin index was evident in the cytoplasm of both glandular (59 ± 55; 55 ± 40) and squamous (15 ± 16; 18 ± 19) mucosa, being significantly higher in the glandular mucosa (P = 0.001; P = 0.041), respectively. In contrast, donkeys showed a higher survivin index in the cytoplasm, but no significant difference was observed between the two types of mucosa (P = 0.506).

Conclusion

Survivin expression in the healthy gastric tissue of equids was localized to the lamina propria and the basal layer of the epithelial layer, predominantly within the cytoplasmic compartment of the cells. However, further techniques are needed to study this protein in Equine Gastric Ulcer Syndrome (EGUS) to assess its potential role in cytoprotection and gastro-adaptation in these species.

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马、驴和骡胃粘膜细胞浆和核Survivin表达的比较研究

背景关于马科动物胃粘膜Survivin蛋白表达的信息很少。该蛋白与调节上皮细胞凋亡和促进有丝分裂的功能有关，被认为是人类和小鼠胃细胞保护机制的一部分，维持粘膜完整性和调节细胞更新。目的研究马、驴、骡等马科动物胃粘膜细胞浆和核Survivin的表达。方法采用组织和免疫组化技术对30只健康胃进行组织学和免疫组化评价。用抗体EP119和聚合物法检测Survivin蛋白。结果免疫组化结果显示，马胃黏膜有survivin的表达。在马和骡子中，腺粘膜（59±55;55±40）和鳞状粘膜（15±16;18±19）的细胞质中均有明显的survivin指数，其中腺粘膜的survivin指数显著高于腺粘膜（P = 0.001; P = 0.041）。相比之下，毛驴在细胞质中表现出更高的survivin指数，但两种黏膜之间无显著差异（P = 0.506）。结论正常海马胃组织中survivin的表达定位于胃固有层和胃上皮基底层，主要表达于胃细胞的细胞质区。然而，需要进一步的技术来研究这种蛋白在马胃溃疡综合征（EGUS）中的潜在作用，以评估其在这些物种的细胞保护和胃适应中的潜在作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Equine Veterinary Science 农林科学-兽医学

CiteScore

2.70

自引率

7.70%

发文量

249

审稿时长

77 days

期刊介绍： Journal of Equine Veterinary Science (JEVS) is an international publication designed for the practicing equine veterinarian, equine researcher, and other equine health care specialist. Published monthly, each issue of JEVS includes original research, reviews, case reports, short communications, and clinical techniques from leaders in the equine veterinary field, covering such topics as laminitis, reproduction, infectious disease, parasitology, behavior, podology, internal medicine, surgery and nutrition.