A metagenomic approach for microbial risk assessment and source attribution in high-risk ports of entry environments

IF 3 Q1 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
Xiaozhou He , Ran Zhang , Jie Dong , Wei Zhen , Li Zhu , Junkai Ren , Xuejun Ma , Feng Wang , Shuang Zhang , Ke Xu , Feng Qiu , Qiudong Su , Jian’an He , Weimin Zhou , Guizhen Wu
{"title":"A metagenomic approach for microbial risk assessment and source attribution in high-risk ports of entry environments","authors":"Xiaozhou He ,&nbsp;Ran Zhang ,&nbsp;Jie Dong ,&nbsp;Wei Zhen ,&nbsp;Li Zhu ,&nbsp;Junkai Ren ,&nbsp;Xuejun Ma ,&nbsp;Feng Wang ,&nbsp;Shuang Zhang ,&nbsp;Ke Xu ,&nbsp;Feng Qiu ,&nbsp;Qiudong Su ,&nbsp;Jian’an He ,&nbsp;Weimin Zhou ,&nbsp;Guizhen Wu","doi":"10.1016/j.bsheal.2025.07.001","DOIUrl":null,"url":null,"abstract":"<div><div>The epidemiological characteristics of emerging infectious disease outbreaks in recent years have underscored the critical importance of controlling imported infectious diseases. In this study, we implemented dynamic tracking of microbial invasions by monitoring environmental microbes at the customs and ports. From July to September 2024, a total of 126 environmental samples were collected from three ports of entry in Shenzhen, China. Metagenomic analysis detected 55 non-viral microbial communities and 12 viral taxa. Among these, 26.8 % of the bacteria, 100 % of the fungi, 71.4 % of the protists, and none of the archaea exhibited potential pathogenic properties. Viruses were the most prevalent, including bacteriophages (100 %), unclassified viruses (96.8 %), giant viruses (27.8 %), fungal viruses (4.8 %), and vertebrate viruses (1.6 %). No statistical differences were observed in viral distribution across areas (<em>χ<sup>2</sup></em> = 18.70, <em>P</em> = 0.541), sites (<em>χ<sup>2</sup></em> = 14.02, <em>P</em> = 0.597), or ports of entry (<em>χ<sup>2</sup></em> = 10.27, <em>P</em> = 0.247). However, viral distribution varied significantly across three sampling months (<em>χ<sup>2</sup></em> = 21.06, <em>P</em> = 0.002), with a higher proportion of giant viruses detected in July. Thirty-nine and forty microorganisms were identified across the six areas and five sites, respectively, with relatively few area/site-specific microorganisms. Four distinct disinfection level zones were categorized: relatively safe zone, less safe zone, general disinfection zone and key disinfection zone. Two strains of viruses with potential pathogenicity were identified: pigeon circovirus and Influenza A virus (H4N2). This study established a metagenomics-based surveillance framework for microbial risk assessment in high-risk port environments and proposed a four-tier disinfection strategy to prioritize high-contact zones. Our findings highlighted environmental metagenomics as a critical complement to traveler screening and provided early warning signals for the prevention and control of imported infectious diseases.</div></div>","PeriodicalId":36178,"journal":{"name":"Biosafety and Health","volume":"7 4","pages":"Pages 228-237"},"PeriodicalIF":3.0000,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biosafety and Health","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2590053625000941","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH","Score":null,"Total":0}
引用次数: 0

Abstract

The epidemiological characteristics of emerging infectious disease outbreaks in recent years have underscored the critical importance of controlling imported infectious diseases. In this study, we implemented dynamic tracking of microbial invasions by monitoring environmental microbes at the customs and ports. From July to September 2024, a total of 126 environmental samples were collected from three ports of entry in Shenzhen, China. Metagenomic analysis detected 55 non-viral microbial communities and 12 viral taxa. Among these, 26.8 % of the bacteria, 100 % of the fungi, 71.4 % of the protists, and none of the archaea exhibited potential pathogenic properties. Viruses were the most prevalent, including bacteriophages (100 %), unclassified viruses (96.8 %), giant viruses (27.8 %), fungal viruses (4.8 %), and vertebrate viruses (1.6 %). No statistical differences were observed in viral distribution across areas (χ2 = 18.70, P = 0.541), sites (χ2 = 14.02, P = 0.597), or ports of entry (χ2 = 10.27, P = 0.247). However, viral distribution varied significantly across three sampling months (χ2 = 21.06, P = 0.002), with a higher proportion of giant viruses detected in July. Thirty-nine and forty microorganisms were identified across the six areas and five sites, respectively, with relatively few area/site-specific microorganisms. Four distinct disinfection level zones were categorized: relatively safe zone, less safe zone, general disinfection zone and key disinfection zone. Two strains of viruses with potential pathogenicity were identified: pigeon circovirus and Influenza A virus (H4N2). This study established a metagenomics-based surveillance framework for microbial risk assessment in high-risk port environments and proposed a four-tier disinfection strategy to prioritize high-contact zones. Our findings highlighted environmental metagenomics as a critical complement to traveler screening and provided early warning signals for the prevention and control of imported infectious diseases.
高风险入境口岸环境中微生物风险评估和来源归因的宏基因组方法
近年来新出现的传染病爆发的流行病学特点突出了控制输入性传染病的极端重要性。在本研究中,我们通过监测海关和港口的环境微生物,实现了微生物入侵的动态跟踪。2024年7月至9月,在中国深圳的三个入境口岸采集了126份环境样本。宏基因组分析检测到55个非病毒微生物群落和12个病毒分类群。其中,26.8%的细菌、100%的真菌、71.4%的原生生物和无一的古细菌表现出潜在的致病特性。病毒最常见,包括噬菌体(100%)、未分类病毒(96.8%)、巨型病毒(27.8%)、真菌病毒(4.8%)和脊椎动物病毒(1.6%)。病毒在地区(χ2 = 18.70, P = 0.541)、地点(χ2 = 14.02, P = 0.597)、入境口岸(χ2 = 10.27, P = 0.247)的分布差异均无统计学意义。但病毒分布在3个采样月份间差异显著(χ2 = 21.06, P = 0.002),其中7月份检测到的巨型病毒比例较高。在6个区域和5个站点中分别鉴定出39种和40种微生物,区域/站点特异性微生物相对较少。将消毒等级划分为相对安全区、较不安全区、一般消毒区和重点消毒区。鉴定出两株具有潜在致病性的病毒:鸽子圆环病毒和甲型流感病毒(H4N2)。本研究建立了基于宏基因组学的高风险港口环境微生物风险评估监测框架,并提出了优先考虑高接触区的四层消毒策略。我们的研究结果强调了环境宏基因组学作为旅行者筛查的重要补充,并为预防和控制输入性传染病提供了早期预警信号。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Biosafety and Health
Biosafety and Health Medicine-Infectious Diseases
CiteScore
7.60
自引率
0.00%
发文量
116
审稿时长
66 days
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信