Morpho-molecular characterization of Agroathelia rolfsii causing leaf blight of sacred lotus (Nelumbo nucifera) in the Andaman and Nicobar Islands

IF 3.3 3区农林科学 Q2 PLANT SCIENCES

Physiological and Molecular Plant Pathology Pub Date : 2025-08-23 DOI:10.1016/j.pmpp.2025.102899

Shivannegowda Mahadevakumar , Attihalli Shivalingegowda Savitha , Chalasani Danteswari , PullabhotlaV.S.R.N. Sarma , Madapati Renuka , Kadaiah Ajithkumar , Madappa Mahesh , Patro Thanuku Samuel Sampath Kumar

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Abstract

Nelumbo nucifera Gaertn., (Nelumbonaceae) is a culturally and medicinally significant, aquatic plant in India, was observed to exhibit a characteristic leaf spot and blight symptoms at the Dhanikhari Experimental Garden cum Arboretum and Interpretation Centre, Forest Department, Shri Vijaya Puram. The disease severity was ranged from 16 % to 23 %. Symptomatic leaf samples were collected, and the associated fungal pathogen was isolated on Potato Dextrose Agar (PDA). Based on a combination of morphological, cultural, and micro-morphological characteristics, the pathogen was identified as Agroathelia rolfsii. The ITS-rDNA region and TEF-1α gene were amplified from genomic DNA of two representative isolates and were sequenced. BLAST analysis revealed high sequence similarity with known A. rolfsii reference sequences from GenBank database. Phylogenetic analysis based on ITS-rDNA confirmed the identity, placing the isolates within a clade containing authenticated A. rolfsii strains. Pathogenicity tests demonstrated symptom development within 3–5 days post-inoculation, and the pathogen was successfully re-isolated, fulfilling Koch's postulates. Based on morphological, molecular, and pathological evidence, this study confirms A. rolfsii as the causal agent of leaf spot and blight in N. nucifera. To the best of our knowledge, this is the first report of A. rolfsii infecting N. nucifera in India.

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安达曼和尼科巴群岛圣莲叶枯病的形态分子特征

荷叶莲。（Nelumbonaceae）是印度一种具有重要文化和药用意义的水生植物，在Shri Vijaya Puram森林部Dhanikhari实验花园兼植物园和解说中心观察到它表现出特有的叶斑病和枯萎症状。疾病严重程度从16%到23%不等。采集有症状的叶片样品，在马铃薯葡萄糖琼脂（PDA）上分离相关真菌病原体。结合形态、培养和微形态特征，鉴定该病原菌为罗氏Agroathelia rolfsi。从两株代表性菌株的基因组DNA中扩增出ITS-rDNA区域和TEF-1α基因，并进行测序。BLAST分析显示与GenBank数据库中已知的A. rolfsii参考序列具有较高的相似性。基于ITS-rDNA的系统发育分析证实了其身份，将分离物置于含有经鉴定的罗尔夫假单胞杆菌菌株的进化支中。致病性测试表明，在接种后3-5天内症状出现，病原体被成功地重新分离，符合科赫的假设。基于形态学、分子和病理学的证据，本研究证实了圆叶蚜是导致白斑病和枯萎病的病原。据我们所知，这是印度首次报告罗尔夫希氏蜱感染北蝽。

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来源期刊

Physiological and Molecular Plant Pathology 生物-植物科学

CiteScore

4.30

自引率

7.40%

发文量

130

审稿时长

38 days

期刊介绍： Physiological and Molecular Plant Pathology provides an International forum for original research papers, reviews, and commentaries on all aspects of the molecular biology, biochemistry, physiology, histology and cytology, genetics and evolution of plant-microbe interactions. Papers on all kinds of infective pathogen, including viruses, prokaryotes, fungi, and nematodes, as well as mutualistic organisms such as Rhizobium and mycorrhyzal fungi, are acceptable as long as they have a bearing on the interaction between pathogen and plant.