Fat body specific ABCG3 accounts for xenobiotic tolerance and is regulated by the homeobox transcription factor Bcd in Spodoptera litura

IF 4 1区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Pesticide Biochemistry and Physiology Pub Date : 2025-08-21 DOI:10.1016/j.pestbp.2025.106650

Lin Liang , Long Jin , Furong Mao , Chengcheng Fan , Haoran Kong , Zixin Zhang , Yiou Pan , Qingli Shang

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Abstract

ATP-binding cassette (ABC) transporters facilitate the transport of various macromolecules within organisms by binding to and hydrolyzing ATP. This study explored the role of SlABCG3 in conferring tolerance to xenobiotics in the globally distributed pest Spodoptera litura. RNA-seq and quantitative PCR (qPCR) results revealed the tissue-specific expression of SlABCG3 in the fat body. Ectopic expression of SlABCG3 in Drosophila melanogaster led to 2.61- and 2.56-fold increases in tolerance to cyantraniliprole and chlorantraniliprole, respectively. Compared with the UAS-SlABCG3 line, the Act5C > UAS-SlABCG3 line presented increased egg production and a lower mortality rate upon exposure to plant secondary metabolites. Furthermore, SlABCG3 knockdown resulted in mortality rates of 70 % and 76.67 % for third-instar larvae of S. litura exposed to cyantraniliprole and chlorantraniliprole, respectively. Additionally, there was a positive regulatory interaction between the tissue-specific transcription factor SlBcd and SlABCG3 in the fat body. Molecular docking results confirmed that SlABCG3 has a strong binding affinity for several of xenobiotics. These results suggest that SlABCG3 may be regulated by SlBcd, which will help us further understand the role of plant secondary metabolites in the mechanism of ABC gene-mediated insecticide tolerance.

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斜纹夜蛾脂肪体特异性ABCG3与外源耐受性有关，并受同源盒转录因子Bcd调控

ATP结合盒（ABC）转运体通过结合和水解ATP促进生物体内各种大分子的运输。本研究探讨了SlABCG3在全球分布的斜纹夜蛾（Spodoptera litura）对外源药物耐受中的作用。RNA-seq和定量PCR （qPCR）结果揭示了SlABCG3在脂肪体中的组织特异性表达。SlABCG3在黑腹果蝇中的异位表达使其对氰虫腈和氯虫腈的耐受性分别增加2.61倍和2.56倍。与UAS-SlABCG3相比，Act5C >； UAS-SlABCG3暴露于植物次生代谢物后，产蛋量增加，死亡率降低。此外，敲除SlABCG3后，暴露于氰虫腈和氯虫腈的夜蛾3龄幼虫的死亡率分别为70%和76.67%。此外，在脂肪体中，组织特异性转录因子SlBcd和SlABCG3之间存在正调控相互作用。分子对接结果证实，SlABCG3对多种外源性药物具有较强的结合亲和力。这些结果表明SlABCG3可能受到SlBcd的调控，这将有助于我们进一步了解植物次生代谢物在ABC基因介导的杀虫剂耐受机制中的作用。

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来源期刊

Pesticide Biochemistry and Physiology 生物-昆虫学

CiteScore

7.00

自引率

8.50%

发文量

238

审稿时长

4.2 months

期刊介绍： Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance. Research Areas Emphasized Include the Biochemistry and Physiology of: • Comparative toxicity • Mode of action • Pathophysiology • Plant growth regulators • Resistance • Other effects of pesticides on both parasites and hosts.