Effects of fucoidan and quercetin on protecting crayfish (Procambarus clarkii) against infection with white spot syndrome virus

IF 2.4 3区生物学 Q1 ZOOLOGY

Journal of invertebrate pathology Pub Date : 2025-08-20 DOI:10.1016/j.jip.2025.108433

Qingri Jin , Xinyu Ji , Xiali Wei , Jing Gong , Fei Zhu

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Abstract

White spot syndrome virus (WSSV) acts as a dangerous pathogen which threats the farming of crustaceans. This study aims to investigate the effect of diet supplemented with fucoidan and quercetin on the immunity and disease resistance of crayfish by studying the apoptosis of hemocytes, crayfish mortality rate and related immune parameters. The feed additive combination of 30 mg/kg fucoidan and 40 mg/kg quercetin (fucoidan + quercetin) significantly improved by 45 % the survival of crayfish (Procambarus clarkii) after infection with WSSV. The fucoidan + quercetin enhanced the total hemocyte counts in crayfish with or without WSSV infection. The combined diet of fucoidan and quercetin has a protective effect on the hemocytes of crayfish, and can reduce the apoptosis of hemocytes after WSSV infection. In addition, the combined diet of fucoidan and quercetin can affect the activities of various immune-related enzymes including superoxide dismutase (SOD), phenoloxidase (PO), catalase (CAT), lysozyme (LZM), acid phosphatase (ACP), and peroxidase (POD), thus modulating the related immune processes. In conclusion, the combination of fucoidan and quercetin can regulate crayfish’s innate immunity and reduce mortality of crayfish after infection with WSSV.

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岩藻聚糖和槲皮素对克氏原螯虾抗白斑综合征病毒感染的保护作用

白斑综合征病毒（WSSV）是一种威胁甲壳类动物养殖的危险病原体。本研究旨在通过研究褐藻聚糖和槲皮素对小龙虾血细胞凋亡、小龙虾死亡率及相关免疫参数的影响，探讨添加褐藻聚糖和槲皮素对小龙虾免疫力和抗病性的影响。30 mg/kg岩藻糖聚糖和40 mg/kg槲皮素（岩藻糖聚糖+槲皮素）的饲料添加剂组合可使感染WSSV的克氏原螯虾（Procambarus clarkii）的存活率显著提高45%。岩藻聚糖+槲皮素能显著提高感染或未感染WSSV的小龙虾的总血细胞计数。褐藻多糖与槲皮素联合饲粮对小龙虾血细胞有保护作用，可减少WSSV感染后小龙虾血细胞的凋亡。此外，岩藻聚糖和槲皮素联合饲粮可影响超氧化物歧化酶（SOD）、酚氧化酶（PO）、过氧化氢酶（CAT）、溶菌酶（LZM）、酸性磷酸酶（ACP）和过氧化物酶（POD）等多种免疫相关酶的活性，从而调节相关免疫过程。综上所述，岩藻聚糖与槲皮素联合使用可调节小龙虾感染WSSV后的先天免疫，降低小龙虾的死亡率。

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来源期刊

Journal of invertebrate pathology 生物-动物学

CiteScore

6.10

自引率

5.90%

发文量

审稿时长

1 months

期刊介绍： The Journal of Invertebrate Pathology presents original research articles and notes on the induction and pathogenesis of diseases of invertebrates, including the suppression of diseases in beneficial species, and the use of diseases in controlling undesirable species. In addition, the journal publishes the results of physiological, morphological, genetic, immunological and ecological studies as related to the etiologic agents of diseases of invertebrates. The Journal of Invertebrate Pathology is the adopted journal of the Society for Invertebrate Pathology, and is available to SIP members at a special reduced price.