Evaluation of the Effects of Riboxin, Copper Chlorophyllin, Indralin, and Combined Application of Glutathione and Ascorbic Acid on the Degree of DNA Damage in the Spleen of Mice under Fractionated Irradiation

Abstract

Using alkaline gel electrophoresis of single cells of the spleen of male ICR (CD-1) mice exposed to five daily exposures to X-ray radiation at a dose of 1.4 Gy, DNA damage was studied on the day of the last irradiation against application of riboxin, or inosine (200 µg/g), copper chlorophyllin (20 µg/g), indralin (50 µg/g), and antioxidants: glutathione (350 µg/g) and ascorbic acid (150 µg/g) together. The 30-day survival of groups of ten mice was evaluated in parallel. The level of DNA damage when using indralin and riboxin did not differ significantly from the intact mice. However, one mouse each from the riboxin and antioxidant groups died. There were no deaths of mice from other groups, including the irradiated control. The use of antioxidants protected DNA to a small extent. Copper chlorophyllin did not protect DNA compared to the irradiated controls.