A Study of the Distribution of Subcellular Structures in Algae Cells Using Optical Laser Tomography

Abstract

Using the developed method of optical laser tomography, the possibility of recording and analyzing the three-dimensional distribution of subcellular organelles in eukaryotic algae (C. rainhardtii) was proved. The method allows one to analyze the redistribution of subcellular organelles (the nucleus and chloroplast) in consecutive slices of one cell in the norm and under the influence of modifiers of its functional state. According to the authors, the proposed approach will allow one not only to study the dynamics of the distribution of intracellular structures (nucleus, thylakoids, mitochondria, etc.), but also changes of a single structure during cell functioning.