Neem (Azadirachta indica) leaf extract mediated synthesis of zinc oxide nanoparticles (ZnO NPs) and their antibacterial activity

IF 4.1 3区材料科学 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY

Nanoscale Research Letters Pub Date : 2025-08-22 DOI:10.1186/s11671-025-04260-4

Elmineh Tsegahun, Muluken Aklilu

{"title":"Neem (Azadirachta indica) leaf extract mediated synthesis of zinc oxide nanoparticles (ZnO NPs) and their antibacterial activity","authors":"Elmineh Tsegahun, Muluken Aklilu","doi":"10.1186/s11671-025-04260-4","DOIUrl":null,"url":null,"abstract":"<div><p>In this study, we prepared zinc oxide nanoparticles using a quick, safe, and cost-effective method by reducing Zn(NO<sub>3</sub>)<sub>2</sub>·6H<sub>2</sub>O solution with Neem (<i>Azadirachta indica</i>) leaf extract. Qualitative phytochemical screening and FT-IR spectroscopy measurements were employed to validate the presence of active biomolecules such as Flavonoids, phenols, alkaloids, terpenes and tannic compounds. FT-IR, UV–Vis, and XRD spectroscopic techniques were utilized to fully analyze the biosynthesized nanoparticles. The spectrum of UV–Visible spectroscopy indicated UV–Vis spectrum of 321 nm. FTIR spectra showed the absorption peak for the stretching vibration of Zn–O at 544 cm<sup>−1</sup>. The results obtained supported the formation of ZnO NPs employing <i>A. indica</i> leaf extract as a reducing and stabilizing agent. X-ray diffraction spectrum analysis was also used to investigate the crystal structure. The particle size of ZnO NPs was calculated using the Scherrer’s equation and the result was found to be 19.16 nm. Furthermore, the antibacterial potential of zinc oxide nanoparticles against two clinical strains of <i>Escherichia coli</i> (<i>E. coli</i>) and <i>Staphylococcus aureus</i> (<i>S. aureus</i>) bacteria was examined by paper disc diffusion method. The result showed a significant inhibition zone of 18 mm against <i>E. coli</i> and an inhibition zone of 15 mm against <i>S. aureus</i>.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":51136,"journal":{"name":"Nanoscale Research Letters","volume":"20 1","pages":""},"PeriodicalIF":4.1000,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1186/s11671-025-04260-4.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nanoscale Research Letters","FirstCategoryId":"88","ListUrlMain":"https://link.springer.com/article/10.1186/s11671-025-04260-4","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

In this study, we prepared zinc oxide nanoparticles using a quick, safe, and cost-effective method by reducing Zn(NO₃)₂·6H₂O solution with Neem (Azadirachta indica) leaf extract. Qualitative phytochemical screening and FT-IR spectroscopy measurements were employed to validate the presence of active biomolecules such as Flavonoids, phenols, alkaloids, terpenes and tannic compounds. FT-IR, UV–Vis, and XRD spectroscopic techniques were utilized to fully analyze the biosynthesized nanoparticles. The spectrum of UV–Visible spectroscopy indicated UV–Vis spectrum of 321 nm. FTIR spectra showed the absorption peak for the stretching vibration of Zn–O at 544 cm⁻¹. The results obtained supported the formation of ZnO NPs employing A. indica leaf extract as a reducing and stabilizing agent. X-ray diffraction spectrum analysis was also used to investigate the crystal structure. The particle size of ZnO NPs was calculated using the Scherrer’s equation and the result was found to be 19.16 nm. Furthermore, the antibacterial potential of zinc oxide nanoparticles against two clinical strains of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) bacteria was examined by paper disc diffusion method. The result showed a significant inhibition zone of 18 mm against E. coli and an inhibition zone of 15 mm against S. aureus.

Graphical Abstract

查看原文本刊更多论文

印楝叶提取物介导氧化锌纳米粒子（ZnO NPs）的合成及其抑菌活性

本研究以印楝叶提取物还原Zn(NO3)2·6H2O溶液为原料，采用快速、安全、经济的方法制备氧化锌纳米颗粒。采用定性植物化学筛选和FT-IR光谱测量来验证活性生物分子的存在，如黄酮类、酚类、生物碱、萜烯和单宁化合物。利用FT-IR， UV-Vis和XRD光谱技术对生物合成的纳米颗粒进行了全面分析。紫外可见光谱为321 nm；FTIR光谱显示Zn-O的拉伸振动吸收峰在544 cm−1处。研究结果支持了以籼稻叶提取物为还原剂和稳定剂制备ZnO纳米粒子的过程。并用x射线衍射光谱分析对晶体结构进行了研究。采用Scherrer方程计算ZnO纳米粒子的粒径，得到ZnO纳米粒子的粒径为19.16 nm。此外，采用纸片扩散法检测氧化锌纳米颗粒对大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus）两种临床菌株的抑菌潜力。结果表明，对大肠杆菌和金黄色葡萄球菌的抑制范围分别为18 mm和15 mm。图形抽象

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Nanoscale Research Letters 工程技术-材料科学：综合

CiteScore

11.30

自引率

0.00%

发文量

110

审稿时长

48 days

期刊介绍： Nanoscale Research Letters (NRL) provides an interdisciplinary forum for communication of scientific and technological advances in the creation and use of objects at the nanometer scale. NRL is the first nanotechnology journal from a major publisher to be published with Open Access.