The Spectral and Kinetic Characteristics of Tryptophan Fluorescence in Human and Bovine Serum Albumin at Different Temperatures

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology

Biophysics Pub Date : 2025-08-22 DOI:10.1134/S0006350925700277

V. Z. Paschenko, V. V. Gorokhov, P. P. Knox, B. N. Korvatovsky, N. P. Grishanova, S. N. Goryachev, A. B. Rubin

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Abstract

The temperature dependence of the duration of tryptophan fluorescence in human and bovine serum albumin in an aqueous solution and glycerol in the temperature range of –170 to 20°C has been studied. A model of forward and reverse electronic transitions in the tryptophan molecule from the excited state to the ground state and to the charge transfer state has been constructed. Three main spectral regions of tryptophan fluorescence with different behaviors of the temperature dependences of transition rates from the excited state of tryptophan to the state with charge transfer were determined. It was found that the dynamics of the hydrogen bonding system in the selected spectral regions had a determining influence on the character of the changes in the duration of tryptophan fluorescence. The nonlinear dependence of intramolecular transition rates on temperature found in this work is determined by the interaction of tryptophan molecules with the microenvironment. The rearrangements in the hydrogen bonding system of albumin protein containing tryptophan molecule have a determining influence on the processes of excitation deactivation in tryptophan.

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人、牛血清白蛋白色氨酸荧光在不同温度下的光谱和动力学特征

在-170 ~ 20℃的温度范围内，研究了人、牛血清白蛋白和甘油中色氨酸荧光持续时间的温度依赖性。建立了色氨酸分子从激发态到基态和电荷转移态的正向和反向电子跃迁模型。确定了色氨酸荧光的三个主要光谱区域，它们具有从色氨酸激发态到电荷转移态的温度依赖性转变速率。研究发现，在选定的光谱区域内，氢键系统的动力学对色氨酸荧光持续时间的变化特征有决定性的影响。本研究中发现的分子内转变速率对温度的非线性依赖是由色氨酸分子与微环境的相互作用决定的。含色氨酸白蛋白分子氢键系统中的重排对色氨酸的激发失活过程具有决定性的影响。

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来源期刊

Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

1.20

自引率

0.00%

发文量

期刊介绍： Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.