Polymorphisms in Hg-related genes and their impact on mercury burden and oxidative stress biomarkers among riverside Amazonian populations

IF 8.1 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Chemosphere Pub Date : 2025-08-20 DOI:10.1016/j.chemosphere.2025.144646

Flora Troina Maraslis , Nathália de Assis Aguilar Duarte , Emilene Arusievicz Nunes , Lindiane Eloisa de Lima , Juliana Mara Serpeloni , Milena Horvat , José L. Domingo , Fernando Barbosa Jr. , Gustavo Rafael Mazzaron Barcelos

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Abstract

Mercury (Hg) is a widespread environmental contaminant, with methylmercury (MeHg) being its most toxic and bioavailable form. MeHg accumulates in aquatic food chains, leading to significant human exposure, particularly in Amazonian riverine populations where fish is a dietary staple. This study examined the impact of genetic polymorphisms on Hg exposure levels and oxidative stress biomarkers in 329 individuals from riverside communities in the Brazilian Amazon.

Hg concentrations in blood, plasma, and hair were measured using inductively coupled plasma mass spectrophotometer, while TaqMan genotyping assays were used to analyze polymorphisms in genes involved in Hg toxicokinetics and toxicodynamics (GSS, GLRX, TXNRD, SELENOP, MT, SLC6, and SLC11). Oxidative stress biomarkers were assessed through spectrophotometric analysis.

Statistical analyses revealed significant genetic associations with Hg levels and oxidative stress markers. Individuals with the TXNRD2 rs5748469 AA genotype had higher blood and plasma Hg concentrations, while SLC6A15 rs11116642 CT carriers had lower Hg levels and increased glutathione peroxidase (GPX) activity. GLRX2 rs912071 CC carriers exhibited reduced GPX activity, particularly among males and non-smokers.

These findings contribute to a better understanding of individual susceptibility to Hg toxicity, particularly in populations chronically exposed to MeHg.

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亚马逊河流域汞相关基因多态性及其对汞负荷和氧化应激生物标志物的影响

汞（Hg）是一种广泛存在的环境污染物，甲基汞（MeHg）是其最具毒性和生物可利用性的形式。甲基汞在水生食物链中积累，导致人类大量接触，特别是在以鱼类为主食的亚马逊河流域人口中。本研究调查了来自巴西亚马逊河沿岸社区的329名个体的遗传多态性对汞暴露水平和氧化应激生物标志物的影响。使用电感耦合等离子体质分光光谱仪测量血液、血浆和头发中的汞浓度，同时使用TaqMan基因分型分析与汞毒性动力学和毒性动力学相关的基因多态性（GSS、GLRX、TXNRD、SELENOP、MT、SLC6和SLC11）。氧化应激生物标志物通过分光光度分析进行评估。统计分析显示与汞水平和氧化应激标志物有显著的遗传关联。携带TXNRD2 rs5748469 AA基因型的个体血液和血浆汞浓度较高，而携带SLC6A15 rs11116642 CT的个体汞水平较低，谷胱甘肽过氧化物酶（GPX）活性升高。GLRX2 rs912071 CC携带者GPX活性降低，尤其是在男性和非吸烟者中。这些发现有助于更好地了解个体对汞毒性的易感性，特别是长期暴露于甲基汞的人群。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemosphere 环境科学-环境科学

CiteScore

15.80

自引率

8.00%

发文量

4975

审稿时长

3.4 months

期刊介绍： Chemosphere, being an international multidisciplinary journal, is dedicated to publishing original communications and review articles on chemicals in the environment. The scope covers a wide range of topics, including the identification, quantification, behavior, fate, toxicology, treatment, and remediation of chemicals in the bio-, hydro-, litho-, and atmosphere, ensuring the broad dissemination of research in this field.