Decoration of silver nanoparticles over Galphimia gracilis leaves extract and assessment of their antioxidant properties

Abstract

This study used a green method to create stable and biocompatible Galphimia gracilis leaves extract-silver nanoparticles (GGLE-AgNPs) by using nontoxic plant extract molecules. The presence of several different components, including as polyphenols, flavonoids, saponins, and tannins was confirmed by the qualitative phytochemical analysis of GGLE. These substances are essential for reducing and capping AgNPs. Uv-visible, FT-IR, optical profilometer, particle size and zeta potential analysis have all been used to describe the shape, size and stability of the produced GGLE-AgNPs. Computational studies by DFT and MC simulation was used to study the interaction between the silver cations and plant extract species. The synthesis of GGLE-AgNPs involved the use of several functional groups of the organic compounds found in GGLE as reducing, capping, and stabilizing agents, according to FT-IR studies. The average particle diameter of the generated GGLE-AgNPs is 328 nm and the zeta potential verified the stability of the GGLE-AgNPs. Spherical, polydispersed AgNPs with a high roughness value was detected by optical profilometer imaging. Antioxidant properties were screened by the DPPH (2,2-diphenyl-1-picrylhydrazyl) and hydrogen peroxide (H₂O₂) method. The antioxidant property's EC50 value was also determined in this investigation. As determined by the DPPH and H₂O₂ method, the samples' antioxidant potential was evaluated. The GGLE showed EC50 values of roughly 90.78 ± 0.09 µg/ml and 116.75 ± 0.15 µg/ml, respectively. In contrast, the GGLE-AgNPs showed higher activity, with EC50 values of 78.37 ± 0.11 µg/ml and 85.42 ± 0.13 µg/ml, respectively.