Yak excreta increases the soil N2O emissions by regulating denitrifying bacterial diversity in the alpine marsh, Southwest China

IF 5 2区农林科学 Q1 SOIL SCIENCE

Applied Soil Ecology Pub Date : 2025-08-21 DOI:10.1016/j.apsoil.2025.106383

Xuelian Guo , Wentao Li , Shanfeng Wang , Xin Fang , Li Chen , Chen Yang , Hang Wang

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Abstract

The impact of livestock excreta on soil N₂O emissions has attracted increasing attention in recent years. However, the mechanism of yak excreta on N₂O emissions of marsh soil has not been elucidated. A laboratory incubation experiment was undertaken to assess the impact of yak excreta inputs on soil N₂O emissions in the alpine marsh and ascertain the crucial distinctions between the consequences of adding yak dung (DA) and urine (UA). The N₂O emission flux and cumulative N₂O emissions from UA and DA were markedly elevated compared with those of the experimental reference cohort during the entire experimental period. While the overall emissions of N₂O from dung were found to surpass those from urine (P < 0.05), after their addition to soils, the cumulative N₂O emission of UA was significantly superior to that of DA (P < 0.05). Dung input exerted a negative priming effect on N₂O emissions in marsh soil, while urine input had a positive priming effect. The addition of yak excreta altered soil pH, moisture, NH₄⁺-N and affected the diversity of nirK and nirS, as well as the activities of urease, N-Acetyl-β-glucosidase (NAG), nitrate reductase, and nitrite reductase (P < 0.05). It can be further found from the results of the structural equation model that the pH, moisture, NH₄⁺-N, nitrite reductase activity, and Shannon indices of nirK and nirS were the main predictors for regulating N₂O emissions in marsh soil. These findings offer essential insights into the mechanisms underlying N₂O emissions associated with the application of yak manure in marsh soil, highlighting the vital function of denitrifying bacteria in alpine marsh ecosystems.

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牦牛粪便通过调节高寒沼泽反硝化细菌多样性增加土壤N2O排放

近年来，家畜排泄物对土壤N₂O排放的影响越来越受到人们的关注。然而，牦牛粪便对沼泽土壤N2O排放的影响机制尚不清楚。通过室内培养试验，评估了牦牛粪便输入对高寒沼泽土壤N₂O排放的影响，并确定了添加牦牛粪便（DA）和尿液（UA）的结果之间的关键区别。在整个实验期间，UA和DA的N2O排放通量和累积N2O排放量均显著高于实验参考队列。粪中N2O的总排放量高于尿中（P < 0.05），而添加到土壤中后，UA的累积N2O排放量显著优于DA （P < 0.05）。粪便输入对沼泽土壤N2O排放具有负启动效应，而尿液输入对沼泽土壤N2O排放具有正启动效应。牦牛排泄物的添加改变了土壤pH、水分、NH4+-N，影响了土壤中nirK和nirS的多样性，以及脲酶、n -乙酰-β-葡萄糖苷酶（NAG）、硝酸盐还原酶和亚硝酸盐还原酶的活性（P < 0.05）。从结构方程模型的结果可以进一步发现，pH、水分、NH4+-N、亚硝酸盐还原酶活性以及nirK和nirS的Shannon指数是调节沼泽土壤N2O排放的主要预测因子。这些发现为了解沼泽土壤中施用牦牛粪相关的N2O排放机制提供了重要见解，突出了反硝化细菌在高寒沼泽生态系统中的重要功能。

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来源期刊

Applied Soil Ecology 农林科学-土壤科学

CiteScore

9.70

自引率

4.20%

发文量

363

审稿时长

5.3 months

期刊介绍： Applied Soil Ecology addresses the role of soil organisms and their interactions in relation to: sustainability and productivity, nutrient cycling and other soil processes, the maintenance of soil functions, the impact of human activities on soil ecosystems and bio(techno)logical control of soil-inhabiting pests, diseases and weeds.