{"title":"Comparative Analysis of Reflectance Confocal Microscopy and Multiphoton Microscopy in Basal Cell Carcinoma Diagnosis.","authors":"Wenwen Zhong, Lingyang Long, Ying Feng, Xiahui Han, Jianxin Chen","doi":"10.1002/jbio.202500216","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Basal cell carcinoma (BCC), the most common skin malignancy, requires early diagnosis for optimal outcomes. This study evaluates the complementary roles of reflectance confocal microscopy (RCM) and multiphoton microscopy (MPM) in BCC characterization.</p><p><strong>Methods: </strong>Forty-seven histopathologically confirmed BCC lesions were analyzed. Preoperative dermoscopy and in vivo RCM imaging were performed; post-excision specimens underwent MPM imaging and H&E correlation.</p><p><strong>Results: </strong>RCM demonstrated superior visualization of tumor nests and vascular patterns, while MPM provided quantitative collagen analysis, revealing significant architectural differences between high- and low-risk subtypes (p < 0.01).</p><p><strong>Conclusion: </strong>The combined approach enhances diagnostic accuracy, with RCM enabling rapid assessment and MPM facilitating risk stratification through collagen metrics.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202500216"},"PeriodicalIF":2.3000,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of biophotonics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/jbio.202500216","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Basal cell carcinoma (BCC), the most common skin malignancy, requires early diagnosis for optimal outcomes. This study evaluates the complementary roles of reflectance confocal microscopy (RCM) and multiphoton microscopy (MPM) in BCC characterization.
Methods: Forty-seven histopathologically confirmed BCC lesions were analyzed. Preoperative dermoscopy and in vivo RCM imaging were performed; post-excision specimens underwent MPM imaging and H&E correlation.
Results: RCM demonstrated superior visualization of tumor nests and vascular patterns, while MPM provided quantitative collagen analysis, revealing significant architectural differences between high- and low-risk subtypes (p < 0.01).
Conclusion: The combined approach enhances diagnostic accuracy, with RCM enabling rapid assessment and MPM facilitating risk stratification through collagen metrics.
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