[Effects of hyperoxia on the expression of hippocampal N-methyl D-aspartate receptor 1 and its synapse-associated molecules in neonatal rats].

Q3 Medicine

中国当代儿科杂志 Pub Date : 2025-08-15 DOI:10.7499/j.issn.1008-8830.2501086

Yi Xiong, Lin Cheng, Na Jiang, Tuan-Mei Wang, Tao Bo

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引用次数: 0

Abstract

Objectives: To investigate the effects of hyperoxia on the expression of N-methyl-D-aspartate receptor 1 (NMDAR1) and its synapse-associated molecules, including cannabinoid receptor 1 (CB1R), postsynaptic density 95 (PSD95), and synapsin (SYN), in the hippocampus of neonatal rats.

Methods: One-day-old Sprague-Dawley neonatal rats were randomly divided into a hyperoxia group and a control group (n=8 per group). The hyperoxia group was exposed to 80% ± 5% oxygen continuously, while the control group was exposed to room air, for 7 days. At 1, 3, and 7 days after hyperoxia exposure, hematoxylin and eosin (HE) staining was used to observe histopathological changes in the brain. The expression levels of NMDAR1, CB1R, PSD95, and SYN proteins and mRNAs in the hippocampus were detected by immunohistochemistry, Western blotting, and quantitative real-time PCR.

Results: After 7 days of hyperoxia exposure, the hyperoxia group showed decreased neuronal density and disordered arrangement in brain tissue. Compared with the control group, after 1 day of hyperoxia exposure, CB1R mRNA and both NMDAR1 and CB1R protein expression in the hyperoxia group were significantly downregulated, while SYN protein expression was significantly upregulated (P<0.05). After 3 days, mRNA expression of NMDAR1, CB1R, and SYN was significantly decreased (P<0.05); NMDAR1 and CB1R protein expression was significantly downregulated (P<0.05), while PSD95 and SYN protein expression was significantly upregulated (P<0.05). After 7 days of hyperoxia, the protein expression of NMDAR1 and CB1R was significantly upregulated (P<0.05).

Conclusions: Continuous hyperoxia exposure induces time-dependent changes in the expression levels of NMDAR1 and its synapse-associated molecules in the hippocampus of neonatal rats.

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高氧对新生大鼠海马n -甲基d -天冬氨酸受体1及其突触相关分子表达的影响。

目的：探讨高氧对新生大鼠海马n -甲基- d -天冬氨酸受体1 （NMDAR1）及其突触相关分子大麻素受体1 （CB1R）、突触后密度95 （PSD95）、突触素（SYN）表达的影响。方法：1日龄Sprague-Dawley新生大鼠随机分为高氧组和对照组（每组8只）。高氧组连续暴露于80%±5%的氧气中，对照组暴露于室内空气中，连续7 d。在高氧暴露后第1、3、7天，采用苏木精伊红（HE）染色观察大鼠脑组织病理变化。采用免疫组织化学、Western blotting和实时荧光定量PCR检测海马组织中NMDAR1、CB1R、PSD95、SYN蛋白和mrna的表达水平。结果：高氧暴露7 d后，高氧组脑组织神经元密度下降，排列紊乱。与对照组相比，高氧暴露1 d后，高氧组CB1R mRNA及NMDAR1和CB1R蛋白表达显著下调，SYN蛋白表达显著上调（ppppp）结论：持续高氧暴露诱导新生大鼠海马NMDAR1及其突触相关分子表达水平发生时间依赖性变化。

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来源期刊

中国当代儿科杂志 Medicine-Pediatrics, Perinatology and Child Health

CiteScore

1.50

自引率

0.00%

发文量

5006

期刊介绍： The Chinese Journal of Contemporary Pediatrics (CJCP) is a peer-reviewed open access periodical in the field of pediatrics that is sponsored by the Central South University/Xiangya Hospital of Central South University and under the auspices of the Ministry of Education of China. It is cited as a source in the scientific and technological papers of Chinese journals, the Chinese Science Citation Database (CSCD), and is one of the core Chinese periodicals in the Peking University Library. CJCP has been indexed by MEDLINE/PubMed/PMC of the American National Library, American Chemical Abstracts (CA), Holland Medical Abstracts (EM), Western Pacific Region Index Medicus (WPRIM), Scopus and EBSCO. It is a monthly periodical published on the 15th of every month, and is distributed both at home and overseas. The Chinese series publication number is CN 43-1301/R；ISSN 1008-8830. The tenet of CJCP is to “reflect the latest advances and be open to the world”. The periodical reports the most recent advances in the contemporary pediatric field. The majority of the readership is pediatric doctors and researchers.