Enhanced meropenem activity and stability following load in Polyvinyl alcohol nanofiber scaffolds with sitagliptin as quorum sensing inhibitor on Pseudomonas aeruginosa.

IF 6.5 3区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of Biological Engineering Pub Date : 2025-08-19 DOI:10.1186/s13036-025-00549-1

Abdelrahman A Mohsen, Taghrid S El-Mahdy, Mohamed Emara, Samar A Salim

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引用次数: 0

Abstract

Background: The increasing resistance of bacteria to conventional antibiotics poses a significant health challenge. Innovative strategies, such as combining antibiotics with agents like quorum sensing inhibitors (QSIs), have been developed to combat this issue. QSIs enhance antibiotic efficacy without inhibiting bacterial growth, minimizing the risk of resistance.

Aims: Evaluate the combined effect of Sitagliptin (STG) as a QSI with Meropenem (MER), fabricate drug-loaded Polyvinyl Alcohol (PVA) nanofibers, and investigate their antimicrobial activity against standard Pseudomonas aeruginosa (PAO1) and carbapenem-resistant P. aeruginosa (CRPA).

Methods: The combinatorial effect was assessed using a checkerboard assay. PVA/STG, PVA/MER, and PVA/STG/MER nanofibers were fabricated with varying concentrations of STG (2, 4, 8 mg/mL) and MER (5, 7, 9 mg/mL) via electrospinning. Characterization was performed using FTIR, XRD, and SEM techniques.

Results: STG significantly reduced the minimum inhibitory concentration (MIC) of MER. The 1:2 STG to MER ratio exhibited the highest antimicrobial activity, achieving a comparable zone of inhibition to the highest concentration of MER while utilizing nearly half the amount of MER. The stability of the loaded scaffolds was maintained over three months at 2-8 °C.

Conclusions: Our results underscore the successful fabrication of nanofiber scaffolds and the effectiveness of STG and MER-loaded nanofibers as promising wound dressings for cutaneous P. aeruginosa infections. This study highlights the potential of our innovative nanofiber system to enhance treatment efficacy against multidrug-resistant bacteria, offering a personalized and rapid response wound dressing solution for medical professionals. Ultimately, it shows promise to improve patient recovery and quality of life while minimizing systemic side effects.

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西格列汀作为铜绿假单胞菌群体感应抑制剂增强聚乙烯醇纳米纤维支架中美罗培南的活性和稳定性。

背景：细菌对常规抗生素的耐药性日益增加，对健康构成了重大挑战。创新的策略，如将抗生素与群体感应抑制剂（qsi）等药物联合使用，已经被开发出来解决这个问题。qsi在不抑制细菌生长的情况下提高抗生素疗效，最大限度地降低耐药风险。目的：评价西格列汀（STG）与美罗培南（MER）作为QSI的联合作用，制备载药聚乙烯醇（PVA）纳米纤维，研究其对标准铜绿假单胞菌（PAO1）和耐碳青霉烯类铜绿假单胞菌（CRPA）的抑菌活性。方法：采用棋盘法评价组合效应。采用静电纺丝法制备了不同浓度STG（2、4、8 mg/mL）和MER（5、7、9 mg/mL）的PVA/STG、PVA/MER和PVA/STG/MER纳米纤维。采用FTIR， XRD和SEM技术进行表征。结果：STG显著降低了MER的最低抑制浓度（MIC）。当STG与MER的比例为1:2时，表现出最高的抗菌活性，达到了与最高MER浓度相当的抑制区，而利用了近一半的MER量。负载支架的稳定性在2-8°C下保持了3个多月。结论：我们的研究结果强调了纳米纤维支架的成功制备以及STG和mer负载纳米纤维作为皮肤铜绿假单胞菌感染的有前途的伤口敷料的有效性。这项研究强调了我们创新的纳米纤维系统在提高治疗多药耐药细菌的疗效方面的潜力，为医疗专业人员提供了个性化和快速反应的伤口敷料解决方案。最终，它有望改善患者的康复和生活质量，同时最大限度地减少全身副作用。

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来源期刊

Journal of Biological Engineering BIOCHEMICAL RESEARCH METHODS-BIOTECHNOLOGY & APPLIED MICROBIOLOGY

CiteScore

7.10

自引率

1.80%

发文量

审稿时长

17 weeks

期刊介绍： Biological engineering is an emerging discipline that encompasses engineering theory and practice connected to and derived from the science of biology, just as mechanical engineering and electrical engineering are rooted in physics and chemical engineering in chemistry. Topical areas include, but are not limited to: Synthetic biology and cellular design Biomolecular, cellular and tissue engineering Bioproduction and metabolic engineering Biosensors Ecological and environmental engineering Biological engineering education and the biodesign process As the official journal of the Institute of Biological Engineering, Journal of Biological Engineering provides a home for the continuum from biological information science, molecules and cells, product formation, wastes and remediation, and educational advances in curriculum content and pedagogy at the undergraduate and graduate-levels. Manuscripts should explore commonalities with other fields of application by providing some discussion of the broader context of the work and how it connects to other areas within the field.