Cellulose nanocomposites with stimulus response and targeted recognition function in cancer cells fluorescence imaging and photodynamic process

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-08-21 DOI:10.1007/s00604-025-07478-2

Ting Chen, Min Zhao, Guizhen Hu, Qiuya Wang, Yanmei Zhou

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引用次数: 0

Abstract

Improving the targeting of nanomedicines in cancer diagnosis and treatment can not only greatly enhance the drug efficacy, but also reduce the systemic toxicity of drugs. The design of cellulose-based nanomaterials with targeted recognition and stimulatory response to overexpressed CD44 receptor and glutathione (GSH) in the cell membrane and cytoplasm of cancer cells, respectively, can significantly improve the targeting effect and reduce the biotoxicity due to the two-site recognition effect. Herein, carboxylated nanocellulose as a carrier to covalently graft with disulfide-modified anthocyanin dye molecules (Cyss) to obtain TOCNC-Cyss, and hyaluronic acid (HA) was assembled on the surface of TOCNC-Cyss to construct cellulose-based nanocomposite TOCNC-Cyss@HA (TCH). TCH targets the CD44 receptor on the surface of cancer cell membranes by HA, and overexpressed glutathione to break the disulfide bond and release fluorescent dyes. TCH can light up cancer cells and generate superoxide anion free radicals under the irradiation of 660 nm laser, induce the apoptosis rate of breast cancer cells to 76%, and have very low toxicity to normal cells. The design of dual-targeting site nanocomposites provides an idea for the application of cellulose in targeted fluorescence imaging and therapy of cancer.

Graphical abstract

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纤维素纳米复合材料在癌细胞荧光成像和光动力过程中具有刺激响应和靶向识别功能

提高纳米药物在癌症诊断和治疗中的靶向性，不仅可以大大提高药物的疗效，还可以降低药物的全身毒性。设计针对癌细胞细胞膜和细胞质中过表达的CD44受体和谷胱甘肽（GSH）分别具有靶向识别和刺激反应的纤维素基纳米材料，可以显著提高靶向效果，降低因两地识别作用而产生的生物毒性。本文以羧化纳米纤维素为载体，与二硫修饰的花青素染料分子（Cyss）共价接枝得到TOCNC-Cyss，并将透明质酸（HA）组装在TOCNC-Cyss表面，构建纤维素基纳米复合材料TOCNC-Cyss@HA （TCH）。TCH通过HA靶向癌细胞细胞膜表面的CD44受体，过表达谷胱甘肽打破二硫键，释放荧光染料。在660 nm激光照射下，TCH能点亮癌细胞，产生超氧阴离子自由基，诱导乳腺癌细胞凋亡率达76%，对正常细胞毒性极低。双靶向位点纳米复合材料的设计为纤维素在肿瘤靶向荧光成像和治疗中的应用提供了思路。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.