Comparative Efficiency of High-Throughput Magnetic Bead Method and Polyethylene Glycol Precipitation for Viral Concentration in Sewage

IF 2.7 2区农林科学 Q2 ENVIRONMENTAL SCIENCES

Food and Environmental Virology Pub Date : 2025-08-20 DOI:10.1007/s12560-025-09662-2

Jingyi Jiang, Li Gong, Ping Yao, Jian Xu, Xujian Mao, Jia Chen, Jinyi Jiang, Xiaoqiang Li, Qiong Li, Fengming Wang

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引用次数: 0

Abstract

Monitoring viral loads in sewage can reflect the prevalence of infections within communities to a certain extent. Methods for concentrating and enriching viruses in sewage are also rapidly evolving. The magnetic bead method has been widely adopted for nucleic acid extraction due to its simplicity and high efficiency. In this study, we designed three pre-treatment procedures (T1: sedimentation for 30 min; T2: low-speed centrifugation at 2500×g for 5 min; T3: high-speed centrifugation at 8000×g for 5 min) to identify the optimal pre-treatment for enhancing viral nucleic acid concentration efficiency using the magnetic bead method. Spiked recovery tests were employed to compare the concentration efficiency of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in sewage between the magnetic bead method and the traditional polyethylene glycol (PEG) precipitation method. Real sewage samples were further used to evaluate both methods for enriching enveloped viruses (SARS-CoV-2, influenza virus) and non-enveloped viruses (Norovirus, Rotavirus, Adenovirus). Results demonstrated that low-speed centrifugation (T2) served as the optimal pre-treatment for the magnetic bead-based concentration. The high-throughput magnetic bead method achieved significantly higher recovery rates for SARS-CoV-2, Norovirus, and Adenovirus compared to PEG precipitation. Thus, the high-throughput magnetic bead method can be widely applied for the concentration and concentration of diverse viruses in sewage.

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高通量磁珠法与聚乙二醇沉淀法测定污水中病毒浓度的比较研究

监测污水中的病毒载量可以在一定程度上反映社区内的感染流行情况。在污水中浓缩和富集病毒的方法也在迅速发展。磁珠法以其简单、高效的特点在核酸提取中得到了广泛的应用。在本研究中，我们设计了三个预处理程序（T1：沉降30 min； T2：在2500×g低速离心5 min； T3：在8000×g高速离心5 min），以确定磁头法提高病毒核酸浓缩效率的最佳预处理。采用加标回收率试验比较磁珠法和传统聚乙二醇沉淀法对污水中SARS-CoV-2的富集效率。利用实际污水样本进一步评价两种富集包膜病毒（SARS-CoV-2、流感病毒）和非包膜病毒（诺如病毒、轮状病毒、腺病毒）的方法。结果表明，低速离心（T2）是磁珠浓缩的最佳前处理方法。与聚乙二醇沉淀法相比，高通量磁珠法对SARS-CoV-2、诺如病毒和腺病毒的回收率显著提高。因此，高通量磁珠法可广泛应用于污水中多种病毒的富集和浓缩。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food and Environmental Virology ENVIRONMENTAL SCIENCES-MICROBIOLOGY

CiteScore

6.50

自引率

2.90%

发文量

审稿时长

1 months

期刊介绍： Food and Environmental Virology publishes original articles, notes and review articles on any aspect relating to the transmission of pathogenic viruses via the environment (water, air, soil etc.) and foods. This includes epidemiological studies, identification of novel or emerging pathogens, methods of analysis or characterisation, studies on survival and elimination, and development of procedural controls for industrial processes, e.g. HACCP plans. The journal will cover all aspects of this important area, and encompass studies on any human, animal, and plant pathogenic virus which is capable of transmission via the environment or food.