Pseudovenmycin, a novel lasso peptide from Streptomyces pseudovenezuelae NA07851

Abstract

Pseudovenmycin (1), a novel type III lasso peptide with a disulfide bond formed by two cysteines, was discovered from Streptomyces pseudovenezuelae NA07851 isolated from a marine sediment collected at the seaside of Fuzhou, Fujian province, China. The biosynthetic gene cluster (BGC) (pvm) responsible for the biosynthesis of pseudovenmycin was successfully identified through heterologous expression in Streptomyces albus J1074 strain and thus its efficient production was achieved. The planar structure of pseudovenmycin was assigned by comprehensive analysis of NMR spectroscopy, LC-MS/MS fragmentation and gene sequence. The absolute configurations of the amino acid residues in this compound were confirmed by Marfey's method. Pseudovenmycin displayed weak inhibitory activity against three tested phytopathogenic fungi.