Distribution of nontuberculous Mycobacteria among presumptive drug resistance tuberculosis patients from a ministry of health drug resistance surveillance program, in western Kenya

IF 2 Q3 INFECTIOUS DISEASES

Journal of Clinical Tuberculosis and Other Mycobacterial Diseases Pub Date : 2025-08-19 DOI:10.1016/j.jctube.2025.100559

Okumu Albert , Odeny Lazarus , Ochieng John Benjamin , Odhiambo Ben , Sitati Ruth , Tonui Joan , Ogoro Jeremiah , Wandiga Steve , Collins Ouma

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引用次数: 0

Abstract

Introduction

Nontuberculous Mycobacteria (NTM) species are emerging pathogens causing Pulmonary diseases with no definitive treatment. Molecular techniques enable characterization and drug resistance profiling, this study sought to determine NTM prevalence, circulating species, and distribution factors among presumptive multidrug-resistant tuberculosis (MDR-TB) patients in western Kenya.

Method

Sputum samples were collected between March through October 2022, and transported for testing at Kenya Medical Research Institute (KEMRI) TB laboratory, in Kisumu. The standard NALC-NaOH MGIT culture technique, smear, HAIN AS/CM and NTM drug resistance were carried out.

Results

Of the 155 specimens analyzed, 106 (68.4 %) were males, 41 (26.5 %) HIV positive, and participants of ages 36–45 years, the majority. An overall NTM prevalence of 99 (63.9 %), of whom 63 (63 %) among males reported. In addition, 11 NTM species identified, with M. intracellulare (44, 44 %).

Conclusions

High prevalence of NTM species was observed among middle-aged males and HIV negative participants, Kisumu led in distribution (29 %) and among HIV positive. The NTM prevalence among smear negative vs smear positive, was significant a p < 0.001, hence adequate TB/HIV integration and management, use of molecular techniques, and accurate identification is critical.

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肯尼亚西部卫生部耐药性监测项目推定耐药结核病患者中非结核分枝杆菌的分布情况

非结核分枝杆菌（NTM）是一种新出现的病原体，可引起肺部疾病，尚无明确的治疗方法。分子技术使表征和耐药谱分析成为可能，本研究试图确定肯尼亚西部推定的耐多药结核病（MDR-TB）患者中NTM的流行、传播种类和分布因素。方法于2022年3月至10月期间收集痰样本，并将其运往基苏木的肯尼亚医学研究所（KEMRI）结核病实验室进行检测。进行标准NALC-NaOH MGIT培养技术、涂片、HAIN AS/CM和NTM耐药试验。结果155份样本中，男性106份（68.4%），HIV阳性41份（26.5%），年龄以36-45岁为主。总体NTM患病率为99例（63.9%），其中63例（63%）为男性。此外，鉴定出11种NTM，其中胞内分枝杆菌占44.44%。结论NTM在中年男性和HIV阴性人群中患病率较高，以Kisumu（29%）和HIV阳性人群中分布最多。涂片阴性和涂片阳性的NTM患病率显著（p < 0.001），因此，充分的结核/艾滋病毒整合和管理、使用分子技术和准确识别至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Clinical Tuberculosis and Other Mycobacterial Diseases Medicine-Pulmonary and Respiratory Medicine

CiteScore

4.00

自引率

5.00%

发文量

审稿时长

30 weeks

期刊介绍： Journal of Clinical Tuberculosis and Mycobacterial Diseases aims to provide a forum for clinically relevant articles on all aspects of tuberculosis and other mycobacterial infections, including (but not limited to) epidemiology, clinical investigation, transmission, diagnosis, treatment, drug-resistance and public policy, and encourages the submission of clinical studies, thematic reviews and case reports. Journal of Clinical Tuberculosis and Mycobacterial Diseases is an Open Access publication.