{"title":"Effects of type 2 diabetes and weight loss on serum CK-18 in MASLD patients","authors":"Shuang Zhao , Jian-He Gan","doi":"10.1016/j.nupar.2025.05.001","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the characteristics of peripheral blood cytokeratin-18 fragments (CK-18) in the development of non-alcoholic fatty liver disease (MASLD), and to find an ideal marker for non-invasive diagnosis of MASLD.</div></div><div><h3>Methods</h3><div>A total of 85 patients with MASLD (abdominal B-ultrasound showed fat deposition) were selected. Serum alanine aminotransferase (AST) and aspartate aminotransferase (ALT) were detected. There were 64 MASLD patients with normal ALT<span> and AST, and 21 MASLD patients with abnormal ALT. Among 64 MASLD patients with normal ALT and AST, 20 patients had type 2 diabetes. Twenty healthy individuals were randomly selected as the control group.</span></div></div><div><h3>Results</h3><div>The level of CK-18 M30 and CK-18 M65 in serum of MASLD patients was higher than that of healthy controls (<em>P</em> <!--><<!--> <!-->0.05). The serum CK-18 M30 and CK-18 M65 of MASLD patients with abnormal ALT was higher than that of patients with normal ALT and AST (<em>P</em> <!--><<!--> <!-->0.05). And serum CK18 M30 and CK-18 M65 in MASLD patients were positively correlated with ALT, but not with AST. Among 64 MASLD patients with normal ALT and AST, there was no statistically significant difference in CK-18 M30 and CK-18 M65 between MASLD patients with and without diabetes. Among all MASLD patients, 11 patients lost more than 5 percent of their weight during the study period. Compared with before weight loss, serum CK-18M30 decreased in the 11 patients after weight loss (<em>P</em> <!--><<!--> <!-->0.05). The decrease of serum CK-18 M65 in the 11 patients was not statistically significant.</div></div><div><h3>Conclusion</h3><div>The level of CK-18 in peripheral blood of MASLD patients is mainly related to serum ALT, but not to diabetes. And weight loss can reduce serum CK-18 M30.</div></div>","PeriodicalId":54702,"journal":{"name":"Nutrition Clinique et Metabolisme","volume":"39 3","pages":"Pages 225-228"},"PeriodicalIF":0.4000,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nutrition Clinique et Metabolisme","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0985056225000470","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}
引用次数: 0
Abstract
Objective
To investigate the characteristics of peripheral blood cytokeratin-18 fragments (CK-18) in the development of non-alcoholic fatty liver disease (MASLD), and to find an ideal marker for non-invasive diagnosis of MASLD.
Methods
A total of 85 patients with MASLD (abdominal B-ultrasound showed fat deposition) were selected. Serum alanine aminotransferase (AST) and aspartate aminotransferase (ALT) were detected. There were 64 MASLD patients with normal ALT and AST, and 21 MASLD patients with abnormal ALT. Among 64 MASLD patients with normal ALT and AST, 20 patients had type 2 diabetes. Twenty healthy individuals were randomly selected as the control group.
Results
The level of CK-18 M30 and CK-18 M65 in serum of MASLD patients was higher than that of healthy controls (P < 0.05). The serum CK-18 M30 and CK-18 M65 of MASLD patients with abnormal ALT was higher than that of patients with normal ALT and AST (P < 0.05). And serum CK18 M30 and CK-18 M65 in MASLD patients were positively correlated with ALT, but not with AST. Among 64 MASLD patients with normal ALT and AST, there was no statistically significant difference in CK-18 M30 and CK-18 M65 between MASLD patients with and without diabetes. Among all MASLD patients, 11 patients lost more than 5 percent of their weight during the study period. Compared with before weight loss, serum CK-18M30 decreased in the 11 patients after weight loss (P < 0.05). The decrease of serum CK-18 M65 in the 11 patients was not statistically significant.
Conclusion
The level of CK-18 in peripheral blood of MASLD patients is mainly related to serum ALT, but not to diabetes. And weight loss can reduce serum CK-18 M30.
期刊介绍:
Nutrition Clinique et Métabolisme is the journal of the French-speaking Society of Enteral and Parenteral Nutrition. Associating clinicians, biologists, pharmacists, and fundamentalists, the articles presented in the journal concern man and animals, and deal with organs and cells. The goal is a better understanding of the effects of artificial nutrition and human metabolism. Original articles, general reviews, update articles, technical notes and communications are published, as well as editorials and case reports.