Telomere length measures in the Northern Ireland cohort for the longitudinal study of ageing (NICOLA).

IF 1.7 Q2 MULTIDISCIPLINARY SCIENCES

BMC Research Notes Pub Date : 2025-08-18 DOI:10.1186/s13104-025-07393-y

Claire Hill, Laura Smyth, Jill Kilner, Katie Quinn, Angie Scott, Charlotte Neville, Frank Kee, Bernadette McGuinness, Amy Jayne McKnight

{"title":"Telomere length measures in the Northern Ireland cohort for the longitudinal study of ageing (NICOLA).","authors":"Claire Hill, Laura Smyth, Jill Kilner, Katie Quinn, Angie Scott, Charlotte Neville, Frank Kee, Bernadette McGuinness, Amy Jayne McKnight","doi":"10.1186/s13104-025-07393-y","DOIUrl":null,"url":null,"abstract":"Objectives: Using blood derived DNA collected from individuals within the Northern Ireland COhort for the Longitudinal study of Ageing (NICOLA), quantitative real-time polymerase chain reaction (RT-PCR) based absolute telomere length measures were derived in triplicate. This data is generated on a subset of participants (n = 2,971) within the NICOLA cohort at Wave 1 baseline. NICOLA commenced Wave 3 of data collection in Autumn 2024.Data description: The NICOLA study recruited approximately 8,500 people from across Northern Ireland, with individuals recruited as representative of the Northern Ireland population. At recruitment, the NICOLA cohort was 45% male and 55% female. Adults were recruited over the age of 50, with approximately 50% of participants aged between 50 and 65, and 10% of participants over the age of 80. Telomere length (TL) values were determined for 2,971individuals (47.7% male) across 33 batches using the Absolute Human Telomere Length Quantification qPCR Assay kit and Roche LightCycler 480 II. Within-batch duplicate measures were established, as were water controls and non-template controls.","PeriodicalId":9234,"journal":{"name":"BMC Research Notes","volume":"18 1","pages":"359"},"PeriodicalIF":1.7000,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12359945/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Research Notes","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s13104-025-07393-y","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Objectives: Using blood derived DNA collected from individuals within the Northern Ireland COhort for the Longitudinal study of Ageing (NICOLA), quantitative real-time polymerase chain reaction (RT-PCR) based absolute telomere length measures were derived in triplicate. This data is generated on a subset of participants (n = 2,971) within the NICOLA cohort at Wave 1 baseline. NICOLA commenced Wave 3 of data collection in Autumn 2024.

Data description: The NICOLA study recruited approximately 8,500 people from across Northern Ireland, with individuals recruited as representative of the Northern Ireland population. At recruitment, the NICOLA cohort was 45% male and 55% female. Adults were recruited over the age of 50, with approximately 50% of participants aged between 50 and 65, and 10% of participants over the age of 80. Telomere length (TL) values were determined for 2,971individuals (47.7% male) across 33 batches using the Absolute Human Telomere Length Quantification qPCR Assay kit and Roche LightCycler 480 II. Within-batch duplicate measures were established, as were water controls and non-template controls.

查看原文本刊更多论文

端粒长度测量在北爱尔兰队列纵向研究衰老（NICOLA）。

目的：使用从北爱尔兰队列中收集的用于衰老纵向研究（NICOLA）的个体的血液来源DNA，基于定量实时聚合酶链反应（RT-PCR）的绝对端粒长度测量一式三份。该数据是在NICOLA队列中第1波基线的参与者子集（n = 2971）中生成的。NICOLA于2024年秋季开始第三波数据收集。数据描述：NICOLA研究从北爱尔兰各地招募了大约8,500人，其中个人被招募为北爱尔兰人口的代表。在招募时，NICOLA队列中45%为男性，55%为女性。成年人年龄在50岁以上，大约50%的参与者年龄在50到65岁之间，10%的参与者年龄在80岁以上。使用绝对人端粒长度定量qPCR试剂盒和罗氏LightCycler 480 II测定了33批2971人（47.7%为男性）的端粒长度（TL）值。在批内建立了重复措施，水控制和非模板控制也是如此。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

BMC Research Notes Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

3.60

自引率

0.00%

发文量

363

审稿时长

15 weeks

期刊介绍： BMC Research Notes publishes scientifically valid research outputs that cannot be considered as full research or methodology articles. We support the research community across all scientific and clinical disciplines by providing an open access forum for sharing data and useful information; this includes, but is not limited to, updates to previous work, additions to established methods, short publications, null results, research proposals and data management plans.