Exogenous Melatonin Inhibits the Expression of GmABI5 and Enhances Drought Resistance in Fodder Soybean Through an ABA-Independent Pathway.

IF 6.3 1区生物学 Q1 PLANT SCIENCES

Plant, Cell & Environment Pub Date : 2025-08-19 DOI:10.1111/pce.70114

Xiashun Liu, Along Chen, Qiyun Wei, Chen Wang, Qianhan Zhao, Qinyi Wang, Xueling Zheng, Tiantian He, Jiayi Qi, Hang Yin, Yulong Lin, Xinying Han, Fuchun Xie, Yajun Chen

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Abstract

Drought is a pivotal abiotic stress that hampers plant growth and development; however, melatonin has emerged as a crucial regulator in mitigating plant responses to such drought-induced stress. By subjecting the plants to drought conditions and applying exogenous melatonin, we found that melatonin significantly improves the drought tolerance of fodder soybean. Remarkably, both stomatal examination and hormone analysis point to a substantial reduction in the endogenous ABA levels following melatonin treatment. Transcriptome analysis revealed significant enrichment of differentially expressed genes in the ABA signalling pathway. The pivotal transcription factor GmABI5 was successfully identified and cloned, with the application of exogenous melatonin notably decreasing the expression of GmABI5. Furthermore, the OE-GmABI5 lines exhibited notably retarded growth, accompanied by a rise in ABA content and an upsurge in the expression of ABA-dependent genes. Contrarily, melatonin treatment led to a complete reversal of these traits in the OE-lines. This study provides new insights and methods for enhancing drought resistance in crops and offers a fresh perspective on the interrelationships between hormones in plants under drought stress.

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外源褪黑素通过aba非依赖性途径抑制饲料大豆GmABI5的表达并增强其抗旱性

干旱是阻碍植物生长发育的关键非生物胁迫；然而，褪黑激素已经成为缓解植物对干旱诱导的应激反应的关键调节因子。通过干旱胁迫和外源褪黑素的应用，我们发现褪黑素显著提高了饲料大豆的耐旱性。值得注意的是，气孔检查和激素分析都指出褪黑激素治疗后内源ABA水平大幅降低。转录组分析显示ABA信号通路中差异表达基因显著富集。成功鉴定并克隆了关键转录因子GmABI5，外源褪黑激素的应用显著降低了GmABI5的表达。此外，OE-GmABI5系表现出明显的生长迟缓，同时ABA含量升高，ABA依赖基因的表达激增。相反，褪黑素治疗导致oe系的这些特征完全逆转。该研究为提高作物抗旱性提供了新的见解和方法，并为干旱胁迫下植物激素相互关系的研究提供了新的视角。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant, Cell & Environment 生物-植物科学

CiteScore

13.30

自引率

4.10%

发文量

253

审稿时长

1.8 months

期刊介绍： Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.