A Multimodal Strategy for Quality Control and Botanical Origin Traceability of Codonopsis Radix based on HPLC Fingerprints With Chemometric Analysis

IF 1.7 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Biomedical Chromatography Pub Date : 2025-08-19 DOI:10.1002/bmc.70185

Yalan Li, Piaopiao Yang, Huixian Qing, Zhiyan Xie, Kexin Zhou, Lili Sun, Meiling Chen, Meng Wang, Xiaoliang Ren

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引用次数: 0

Abstract

Codonopsis Radix (CR) is widely used in the food and pharmaceutical industries. However, the existence of three botanical origins in CR makes it challenging to ensure the quality of CR in the market. In this study, a method was developed to identify and analyze CR from different botanical origins, and their signature components were quantitatively compared. HPLC fingerprints of 55 CR batches demonstrated similarities ranging from 0.707 to 0.994. Chemometrics was used to analyze the different botanical origins of CR and identify significant components affecting their classification. Hierarchical cluster analysis (HCA) and principal component analysis (PCA) indicated that 55 CR batches could be divided into three groups according to their botanical origins. Partial least squares discriminant analysis (PLS-DA) was applied to confirm classification results and obtain the chemical markers. In addition, the reliability of the markers was revalidated by counter propagation artificial neural networks (CP-ANN). The content determination results showed significant differences in the contents of adenosine, eleutheroside B, tangshenoside I, and lobetyolin in the three kinds of CR. The methods and results of this study provide a reliable basis for the identification and rational utilization of CR.

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基于HPLC指纹图谱和化学计量分析的党参质量控制和植物来源溯源的多模式策略

党参（Codonopsis Radix， CR）广泛应用于食品和制药行业。然而，由于CR中存在三种植物源，因此很难保证CR在市场上的质量。本研究建立了一种鉴定和分析不同植物源CR的方法，并对其特征成分进行了定量比较。55个CR批次的HPLC指纹图谱相似度在0.707 ~ 0.994之间。利用化学计量学分析了不同植物来源的CR，并确定了影响其分类的重要成分。层次聚类分析（HCA）和主成分分析（PCA）表明，55个CR批次根据植物来源可分为3类。采用偏最小二乘判别分析（PLS-DA）对分类结果进行确认，获得化学标记。此外，通过反向传播人工神经网络（CP-ANN）对标记的可靠性进行了再验证。含量测定结果显示，三种药材中腺苷、刺五苦苷B、汤参苷I、枇杷苷的含量差异有统计学意义，本研究的方法和结果为药材的鉴定和合理利用提供了可靠的依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomedical Chromatography 生物-分析化学

CiteScore

3.60

自引率

5.60%

发文量

268

审稿时长

2.3 months

期刊介绍： Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.