In vivo anti-plasmodial activity of alpha-onocerin and artesunate combination against Plasmodium berghei infected mice

IF 1.3 Q3 PHARMACOLOGY & PHARMACY

Advances in Traditional Medicine Pub Date : 2025-01-07 DOI:10.1007/s13596-024-00812-8

Jacob Hammurabi Kwansah-Obresi, Aliu Moomin, Arnold Donkor Forkuo, Aaron Opoku Antwi, John Nii Adotey Addotey, Paa Kofi Tawiah Adu-Gyamfi, Abubakar Ibn Sidik, Kwesi Boadu Mensah

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Abstract

Alpha-onocerin is a triterpenoid derived from Huperzia phlegmaria which is used in ethnomedicine for the treatment of fever, headaches, pains and malaria. This study was conducted to evaluate the safety, antipyretic and anti-plasmodial activity of alpha-onocerin and artesunate (ART) co-administration in ICR mice for use in traditional medicine.

The anti-plasmodial effects of alpha-onocerin (10, 30, 100, 300 mg/kg) and ART (1, 2, 4, 8, 16 mg/kg) were assessed in P. berghei-infected mice. Alpha-onocerin /ART were administered with a fixed dose combination of their median effective doses (ED_50s) to determine the experimental ED₅₀ (Z_exp). An isobologram was developed to identify the nature of the interaction by comparing Z_exp with the theoretical ED₅₀ (Z_add).

Alpha-onocerin (300 mg/kg) showed a similar chemosuppression (93.51 ± 2.15%) to ART (2 mg/kg, i.p.) of 97.02 ± 0.27% in the 4-day suppressive test as well as in the prophylactic test with chemosuppression at 54.94% and 69.76% for alpha-onocerin (300 mg/kg) and artesunate (2 mg/kg, i.p.) respectively. All doses of alpha-onocerin significantly (p < 0.05) reduced pyrexia in 1 h and 2 h after their administration in the baker’s yeast test. ED₅₀s for ART and alpha-onocerin were 1.33 ± 0.11 and 13.64 ± 0.22 mg/kg, respectively. The Z_add, Z_exp and interaction index for alpha-onocerin /ART co-administration were 7.49 ± 3.46, 1.61 ± 0.78 and 0.22 respectively. The Z_exp for alpha-onocerin /ART laid below the additive isobole indicating significant (p < 0.001) synergistic activity with ART.

Alpha-onocerin showed analgesic effects, antipyretic and synergistic anti-plasmodial effects in P. berghei-infected mice.

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α -蛇麻素和青蒿琥酯联合用药对伯氏疟原虫感染小鼠的体内抗疟原虫活性

α -蛇麻素是一种从痰石杉中提取的三萜，在民族医学中用于治疗发烧、头痛、疼痛和疟疾。本研究旨在评价传统药物治疗ICR小鼠时α -蛇麻素与青蒿琥酯（ART）共给药的安全性、解热和抗疟原虫活性。研究了α -壬烯醛（10、30、100、300 mg/kg）和ART（1、2、4、8、16 mg/kg）对伯氏疟原虫感染小鼠的抗疟原虫作用。将α -onocerin /ART以其中位有效剂量（ED50）的固定剂量组合给药，以测定实验ED50 （Zexp）。通过比较Zexp和理论ED50 (Zadd)，开发了一个等线图来确定相互作用的性质。在4天的抑制试验中，α -鱼麻素（300 mg/kg）和青蒿素（2 mg/kg, i.p）的化学抑制率为97.02±0.27%，与α -鱼麻素（300 mg/kg）和青蒿素（2 mg/kg, i.p）的化学抑制率分别为54.94%和69.76%。在面包酵母试验中，所有剂量的α -茴香素在给药后1 h和2 h显著降低了发热（p < 0.05）。ART和α -onocerin的ed50分别为1.33±0.11和13.64±0.22 mg/kg。α -onocerin与ART共给药的Zadd、Zexp和相互作用指数分别为7.49±3.46、1.61±0.78和0.22。α -蛇麻素/ART的Zexp位于可加性等孔下方，表明与ART具有显著的协同作用（p < 0.001）。α -蛇麻素对伯氏杆菌感染小鼠具有镇痛、解热和协同抗疟原虫作用。

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来源期刊

Advances in Traditional Medicine PHARMACOLOGY & PHARMACY-

CiteScore

4.30

自引率

0.00%

发文量

期刊介绍： Advances in Traditional Medicine (ADTM) is an international and peer-reviewed journal and publishes a variety of articles including original researches, reviews, short communications, and case-reports. ADTM aims to bridging the gap between Traditional knowledge and medical advances. The journal focuses on publishing valid, relevant, and rigorous experimental research and clinical applications of Traditidnal Medicine as well as medical classics. At the same time, the journal is devoted to communication among basic researcher and medical clinician interested in the advancement of Traditional Medicine. Topics covered by the journal are: Medical Classics & History; Biomedical Research; Pharmacology & Toxicology of Natural Products; Acupuncture & Moxibustion; Sasang Constitutional Medicine; Diagnostics and Instrumental Development; Clinical Research. ADTM is published four times yearly. The publication date of this journal is 30th March, June, September, and December.