Effects of dietary zearalenone intake on oocyte quality for in vitro embryo production and on serum anti-Müllerian hormone and inflammatory marker levels, following ovum pick-up in Japanese Black donor cows

IF 3.3 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science Pub Date : 2025-08-15 DOI:10.1016/j.anireprosci.2025.107970

Shogo Hashimoto , Hiroshi Hasunuma , Katsuki Toda , Hinako Fujisaki , Miyuki Arai , Daisaku Matsumoto , Osamu Yamato , Oky Setyo Widodo , Masayasu Taniguchi , Mitsuhiro Takagi

{"title":"Effects of dietary zearalenone intake on oocyte quality for in vitro embryo production and on serum anti-Müllerian hormone and inflammatory marker levels, following ovum pick-up in Japanese Black donor cows","authors":"Shogo Hashimoto , Hiroshi Hasunuma , Katsuki Toda , Hinako Fujisaki , Miyuki Arai , Daisaku Matsumoto , Osamu Yamato , Oky Setyo Widodo , Masayasu Taniguchi , Mitsuhiro Takagi","doi":"10.1016/j.anireprosci.2025.107970","DOIUrl":null,"url":null,"abstract":"<div><div>In Japanese Black (JB) cattle, the number and percentage of transferable in vitro fertilization (IVF) embryos after oocyte collection are essential for the economic success of embryo production. Anti-Müllerian hormone (AMH) is a useful predictive marker for the number of cumulus-oophorus complexes (COCs) obtained through transvaginal oocyte retrieval. We aimed to examine the relationship of AMH levels, serum amyloid A (SAA) levels, and albumin/globulin (A/G) ratios, which are inflammatory markers, and urinary zearalenone (ZEN) levels, which reflect dietary ZEN intake, with the number and quality of oocytes recovered after ovum pickup (OPU) in JB donor cows as well as the number and rate of transferable IVF embryos. Oocytes obtained after OPU were fertilized in vitro. Additionally, blood and urine samples were collected at the time of OPU to assess serum AMH and SAA levels as well as urinary ZEN levels. The number of recovered oocytes (<em>R</em> = 0.745, <em>P</em> = 0.000) and transferable embryos was positively correlated with AMH levels (<em>R</em> = 0.704, <em>P</em> = 0.000) and negatively correlated with gamma-globulin levels. Furthermore, the percentage of transferable embryos per total number of embryos was positively correlated with urinary ZEN levels (<em>P</em> = 0.027). In conclusion, AMH levels can inform the selection of JB donor cows for OPU. Moreover, substandard ZEN contamination of dietary feed may not affect embryo production following OPU; instead, it may result in an increased number of transferable embryos, and in turn facilitate oocyte nuclear and cytoplasmic maturation during follicle development.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"280 ","pages":"Article 107970"},"PeriodicalIF":3.3000,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal Reproduction Science","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S037843202500209X","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}

引用次数: 0

Abstract

In Japanese Black (JB) cattle, the number and percentage of transferable in vitro fertilization (IVF) embryos after oocyte collection are essential for the economic success of embryo production. Anti-Müllerian hormone (AMH) is a useful predictive marker for the number of cumulus-oophorus complexes (COCs) obtained through transvaginal oocyte retrieval. We aimed to examine the relationship of AMH levels, serum amyloid A (SAA) levels, and albumin/globulin (A/G) ratios, which are inflammatory markers, and urinary zearalenone (ZEN) levels, which reflect dietary ZEN intake, with the number and quality of oocytes recovered after ovum pickup (OPU) in JB donor cows as well as the number and rate of transferable IVF embryos. Oocytes obtained after OPU were fertilized in vitro. Additionally, blood and urine samples were collected at the time of OPU to assess serum AMH and SAA levels as well as urinary ZEN levels. The number of recovered oocytes (R = 0.745, P = 0.000) and transferable embryos was positively correlated with AMH levels (R = 0.704, P = 0.000) and negatively correlated with gamma-globulin levels. Furthermore, the percentage of transferable embryos per total number of embryos was positively correlated with urinary ZEN levels (P = 0.027). In conclusion, AMH levels can inform the selection of JB donor cows for OPU. Moreover, substandard ZEN contamination of dietary feed may not affect embryo production following OPU; instead, it may result in an increased number of transferable embryos, and in turn facilitate oocyte nuclear and cytoplasmic maturation during follicle development.

查看原文本刊更多论文

日粮玉米赤霉烯酮摄入量对日本黑供牛体外胚胎生产卵母细胞质量及取卵后血清抗勒氏杆菌激素和炎症标志物水平的影响

在日本黑牛（JB）中，卵母细胞收集后可移植体外受精（IVF）胚胎的数量和百分比对胚胎生产的经济成功至关重要。抗勒氏杆菌激素（AMH）是经阴道卵母细胞回收获得的卵丘-卵巢复合体（COCs）数量的有效预测指标。我们的目的是研究AMH水平、血清淀粉样蛋白A （SAA）水平、白蛋白/球蛋白（A/G）比率（炎症标志物）和尿玉米赤霉烯酮（ZEN）水平（反映饲粮ZEN摄入量）与JB供体奶牛取卵后恢复的卵母细胞数量和质量（OPU）以及可移植体外受精胚胎的数量和率之间的关系。OPU后获得的卵母细胞体外受精。此外，在OPU时采集血液和尿液样本，评估血清AMH和SAA水平以及尿液ZEN水平。恢复卵母细胞数（R = 0.745, P = 0.000）和可移植胚胎数与AMH水平呈正相关（R = 0.704, P = 0.000），与γ -球蛋白水平负相关。可移植胚胎占胚胎总数的比例与尿ZEN水平呈正相关（P = 0.027）。综上所述，AMH水平可以指导供体奶牛的选择。此外，不合格的日粮ZEN污染可能不会影响OPU后的胚胎生产；相反，它可能导致可移植胚胎数量的增加，进而促进卵泡发育过程中卵母细胞核和细胞质的成熟。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Animal Reproduction Science 农林科学-奶制品与动物科学

CiteScore

4.50

自引率

9.10%

发文量

136

审稿时长

54 days

期刊介绍： Animal Reproduction Science publishes results from studies relating to reproduction and fertility in animals. This includes both fundamental research and applied studies, including management practices that increase our understanding of the biology and manipulation of reproduction. Manuscripts should go into depth in the mechanisms involved in the research reported, rather than a give a mere description of findings. The focus is on animals that are useful to humans including food- and fibre-producing; companion/recreational; captive; and endangered species including zoo animals, but excluding laboratory animals unless the results of the study provide new information that impacts the basic understanding of the biology or manipulation of reproduction. The journal''s scope includes the study of reproductive physiology and endocrinology, reproductive cycles, natural and artificial control of reproduction, preservation and use of gametes and embryos, pregnancy and parturition, infertility and sterility, diagnostic and therapeutic techniques. The Editorial Board of Animal Reproduction Science has decided not to publish papers in which there is an exclusive examination of the in vitro development of oocytes and embryos; however, there will be consideration of papers that include in vitro studies where the source of the oocytes and/or development of the embryos beyond the blastocyst stage is part of the experimental design.