Arabidopsis PROTEIN S-ACYL TRANSFERASE4 mediates root hair growth angle and morphology

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2025-08-16 DOI:10.1016/j.plantsci.2025.112723

Chong Feng , Zhiyuan Wan

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引用次数: 0

Abstract

Plants absorb water and nutrients from the soil through their root systems, with root hair cells playing a critical role in this process. Root hair growth is regulated not only by genetic factors but also by environmental cues. Current descriptions of root hair phenotypes primarily focus on root hair density and length. Our previous studies have shown that PAT4 regulates root hair elongation by mediating the plasma membrane localization of ROP2. In this study, we found that loss of PAT4 function leads to a smaller angle between root hairs and the primary root plane, accompanied by abnormal root hair morphology, including branching, upturning, and basal swelling. Conversely, PAT4 overexpression resulted in a significant increase in the root hair growth angle compared to wild-type plants. Exogenous application of auxin exacerbated the morphological abnormalities of root hairs in pat4 mutants. Genetic analysis revealed an additive effect between myosin XIK and PAT4 in regulating root hair elongation. Additionally, although PAT16 is expressed in root hairs and its protein localizes to the Golgi apparatus, phenotypic analysis showed that PAT16 does not participate in root hair growth regulation. Collectively, these findings demonstrate that PAT4 is involved in regulating root hair morphogenesis. Moreover, root hair growth angle provides a new dimension for phenotypic analysis of root hairs.

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拟南芥蛋白S-ACYL TRANSFERASE4介导根毛生长角度和形态

植物通过根系从土壤中吸收水分和养分，根毛细胞在这一过程中起着至关重要的作用。根毛的生长不仅受遗传因素的影响，还受环境因素的影响。目前对根毛表型的描述主要集中在根毛密度和长度上。我们之前的研究表明，PAT4通过介导ROP2的质膜定位来调节根毛伸长。在本研究中，我们发现PAT4功能缺失导致根毛与主根平面夹角变小，并伴有根毛形态异常，包括分枝、上翻、基底肿胀等。相反，与野生型植物相比，PAT4过表达导致根毛生长角度显著增加。外源应用生长素加剧了pat4突变体根毛形态的异常。遗传分析表明，肌球蛋白XIK和PAT4在调节根毛伸长方面具有加性效应。此外，尽管PAT16在根毛中表达，其蛋白定位于高尔基体，但表型分析显示PAT16不参与根毛生长调节。综上所述，这些发现表明PAT4参与了根毛形态发生的调控。根毛生长角度为根毛表型分析提供了一个新的维度。

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.