Genome wide association mapping reveals genetic loci and candidate genes for seedling stage drought tolerance in lentil (Lens culinaris)

IF 4.5 Q1 PLANT SCIENCES

Current Plant Biology Pub Date : 2025-08-05 DOI:10.1016/j.cpb.2025.100531

Neteti Siddartha Kumar , Renu Pandey , Anjali Anand , Amit Kumar Singh , Muraleedhar S. Aski , Gyan Prakash Mishra , Harsh Kumar Dikshit , Mahesh Rao , R.S. Bana , Shiv Kumar , Viswanathan Chinnusamy , Ruchi Bansal

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Abstract

Lentil (Lens culinaris) is a very important cold-season nutritious legume crop. The crop faces intermittent drought in South Asian countries and terminal drought in West Asian and North African Mediterranean regions causing adverse impact on lentil productivity. The present study aimed to evaluate a diverse lentil panel (243 genotypes) under irrigated and drought conditions at seedling stage and to identify significant marker trait associations for drought tolerance traits. Drought stress was imposed by restricting the pre-sowing irrigation. A total of 18 different morpho-physiological traits including root (length, surface area, volume, tips and forks), physiological (germination percentage, NDVI, canopy temperature) and growth (seedling vigor, plant biomass) traits were recorded among the lentil genotypes in both control and stress conditions. All the traits except canopy temperature were found to be significantly reduced under stress. Principal component analysis explained 56.3 % variation in control and 60.7 % variation in drought condition. Shoot dry weight had significant correlation to NDVI, shoot branching, primary and total root length, and root length density. Genotypes IC560032, IC560246, P3227, IC560051, and IG134349 were identified as drought-tolerant using SSI (<0.5). Association mapping analysis identified 65 and 71 non-overlapping distinct SNPs significantly associated with all traits under control and drought conditions, respectively. Putative candidate genes encoding legumain-like cysteine endopeptidase, L-ascorbate oxidase, and auxin-responsive proteins were involved in the regulation of key drought tolerance associated traits like germination percentage, root length, seedling vigor respectively. These findings highlight the potential of lentil germplasm for drought resilience and provide a valuable genetic resource for breeding high-yielding, stress-tolerant varieties.

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小扁豆（Lens culinaris）苗期抗旱性遗传位点和候选基因的全基因组关联定位

小扁豆（Lens culinaris）是一种非常重要的冷季营养豆科作物。该作物在南亚国家面临间歇性干旱，在西亚和北非地中海地区面临终末干旱，对扁豆生产力造成不利影响。本研究旨在评估一个不同的小扁豆群体（243个基因型）在苗期灌溉和干旱条件下的耐旱性，并确定显著的标记性状相关性。通过限制播前灌溉施加干旱胁迫。在对照和胁迫条件下，共记录了18个不同的形态生理性状，包括根（长度、表面积、体积、尖端和分叉）、生理（发芽率、NDVI、冠层温度）和生长（幼苗活力、植株生物量）。除冠层温度外，其余性状均显著降低。主成分分析解释了对照和干旱条件下56.3% %和60.7 %的变异。地上部干重与NDVI、地上部分枝、主根长和总根长、根长密度呈极显著相关。基因型IC560032、IC560246、P3227、IC560051和IG134349通过SSI （<0.5）鉴定为耐旱基因型。关联图谱分析发现，在对照和干旱条件下，65个和71个非重叠的不同snp与所有性状均显著相关。编码豆科类半胱氨酸内肽酶、l -抗坏血酸氧化酶和生长素响应蛋白的候选基因分别参与了发芽率、根长、幼苗活力等关键抗旱相关性状的调控。这些发现突出了扁豆种质的抗旱潜力，为培育高产耐旱品种提供了宝贵的遗传资源。

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来源期刊

Current Plant Biology Agricultural and Biological Sciences-Plant Science

CiteScore

10.90

自引率

1.90%

发文量

审稿时长

50 days

期刊介绍： Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.