Plasma-Derived Extracellular Vesicle-Propagated microRNA From Aortic Stenosis Patients Render Pro-Calcifying Effects on Valve Interstitial Cells

IF 8.4 1区医学 Q1 CARDIAC & CARDIOVASCULAR SYSTEMS

JACC: Basic to Translational Science Pub Date : 2025-08-20 DOI:10.1016/j.jacbts.2025.101327

Avinash B. Khandagale PhD , Padraic Corcoran PhD , Yuzhen Dan PhD , Anders Isaksson PhD , Stefan James MD, PhD , Agneta Siegbahn MD, PhD , Christina Christersson MD, PhD

引用次数: 0

Abstract

Extracellular vesicles (EVs) and microRNAs (miRs) have been found to be differently expressed in patients with aortic valve stenosis (AS). Here, we profiled the expression of miRs associated with circulating EVs from severe AS patients and found several altered miRs compared with healthy counterparts. EVs from AS patients induced calcification through expression of the pro-osteogenic genes osteocalcin and osteopontin with corresponding proteins. The expression levels of miR-455-3p and miR-103a-3p were found correlated to the calcification of VICs. Alteration in these miR abrogated osteogenic differentiation of VICs. Additionally, BMP4 and transcriptional factor RUNX2 were found affected by these miRs.

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主动脉狭窄患者血浆来源的细胞外小泡增殖microRNA对瓣膜间质细胞具有促进钙化的作用

发现细胞外囊泡（EVs）和microRNAs （miRs）在主动脉瓣狭窄（AS）患者中表达不同。在这里，我们分析了严重AS患者与循环ev相关的miRs表达，发现与健康患者相比，miRs发生了一些变化。AS患者的EVs通过表达促成骨基因骨钙素和骨桥蛋白及其相应蛋白诱导钙化。发现miR-455-3p和miR-103a-3p的表达水平与vic的钙化相关。这些miR的改变消除了vic的成骨分化。此外，发现BMP4和转录因子RUNX2受到这些miRs的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

JACC: Basic to Translational Science CARDIAC & CARDIOVASCULAR SYSTEMS-

CiteScore

14.20

自引率

1.00%

发文量

161

审稿时长

16 weeks

期刊介绍： JACC: Basic to Translational Science is an open access journal that is part of the renowned Journal of the American College of Cardiology (JACC). It focuses on advancing the field of Translational Cardiovascular Medicine and aims to accelerate the translation of new scientific discoveries into therapies that improve outcomes for patients with or at risk for Cardiovascular Disease. The journal covers thematic areas such as pre-clinical research, clinical trials, personalized medicine, novel drugs, devices, and biologics, proteomics, genomics, and metabolomics, as well as early phase clinical trial methodology.