Green synthesis of silver nanoparticles using Azadirachta indica (Neem) fruit pulp extract and their antioxidant, antibacterial, and anticancer activity

Bangaru Babu Alampally , Y Subba Rao , Sravanthi Pidamarthi , Sankara Rao Miditana , Pulsingh Dhanavath , Durga Bhavani A.K.
{"title":"Green synthesis of silver nanoparticles using Azadirachta indica (Neem) fruit pulp extract and their antioxidant, antibacterial, and anticancer activity","authors":"Bangaru Babu Alampally ,&nbsp;Y Subba Rao ,&nbsp;Sravanthi Pidamarthi ,&nbsp;Sankara Rao Miditana ,&nbsp;Pulsingh Dhanavath ,&nbsp;Durga Bhavani A.K.","doi":"10.1016/j.scenv.2025.100282","DOIUrl":null,"url":null,"abstract":"<div><div>In this research, silver nanoparticles (AgNPs) were synthesized using <em>Azadirachta indica</em> (Neem) fruit pulp extract as a natural reducing agent. The synthesized AgNPs were characterized by the TEM, AFM, XRD, DLS, FTIR, and UV–visible spectral analysis. Visual confirmation of AgNP synthesis was indicated by the appearance of a reddish-brown color, further supported by a UV–visible SPR band at approximately 453 nm. FTIR analysis supported the formation and stabilization of the nanoparticles, attributed to the phytochemicals present in the flower extract. The AgNPs were characterized by XRD analysis that confirmed the FCC crystalline nature of the AgNPs with an average crystalline size of 20.8 nm. EDX confirmed the presence of elemental silver. HRTEM images showed that the AgNPs are nearly spherical with an average size of 31.5 nm. The high negative potential value of AgNPs (−36.7 mV) was evident from DLS studies. AFM data revealed that the particles were agglomerated and spherical in shape, with less than 40 nm. The AgNPs also exhibited good antioxidant activities against DPPH radical with 66.72 % radical scavenging ability. The biosynthesized AgNPs demonstrated notable anticancer activity against burst cancer cell lines (77.28 µg/mL in the MTT assay) and demonstrated good antibacterial activities against gram-negative bacteria and gram-positive bacteria with a Zone of inhibition ranging from 8 to 16 mm. This study demonstrates the synthesis of AgNPs and their promising in biomedical applications, and the benefits of using plant extracts to prepare green nanoparticles.</div></div>","PeriodicalId":101196,"journal":{"name":"Sustainable Chemistry for the Environment","volume":"11 ","pages":"Article 100282"},"PeriodicalIF":0.0000,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sustainable Chemistry for the Environment","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S294983922500077X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

In this research, silver nanoparticles (AgNPs) were synthesized using Azadirachta indica (Neem) fruit pulp extract as a natural reducing agent. The synthesized AgNPs were characterized by the TEM, AFM, XRD, DLS, FTIR, and UV–visible spectral analysis. Visual confirmation of AgNP synthesis was indicated by the appearance of a reddish-brown color, further supported by a UV–visible SPR band at approximately 453 nm. FTIR analysis supported the formation and stabilization of the nanoparticles, attributed to the phytochemicals present in the flower extract. The AgNPs were characterized by XRD analysis that confirmed the FCC crystalline nature of the AgNPs with an average crystalline size of 20.8 nm. EDX confirmed the presence of elemental silver. HRTEM images showed that the AgNPs are nearly spherical with an average size of 31.5 nm. The high negative potential value of AgNPs (−36.7 mV) was evident from DLS studies. AFM data revealed that the particles were agglomerated and spherical in shape, with less than 40 nm. The AgNPs also exhibited good antioxidant activities against DPPH radical with 66.72 % radical scavenging ability. The biosynthesized AgNPs demonstrated notable anticancer activity against burst cancer cell lines (77.28 µg/mL in the MTT assay) and demonstrated good antibacterial activities against gram-negative bacteria and gram-positive bacteria with a Zone of inhibition ranging from 8 to 16 mm. This study demonstrates the synthesis of AgNPs and their promising in biomedical applications, and the benefits of using plant extracts to prepare green nanoparticles.
利用印楝果果肉提取物及其抗氧化、抗菌、抗癌活性合成绿色纳米银
本研究以印楝果果肉提取物为天然还原剂,合成了银纳米颗粒(AgNPs)。通过TEM、AFM、XRD、DLS、FTIR和紫外可见光谱对合成的AgNPs进行了表征。AgNP合成的视觉确认是由红棕色的外观表明,进一步由紫外可见SPR波段支持,大约453 nm。FTIR分析支持纳米颗粒的形成和稳定,归因于花提取物中存在的植物化学物质。通过XRD对AgNPs进行了表征,证实了AgNPs的FCC结晶性质,平均晶粒尺寸为20.8 nm。EDX证实了元素银的存在。HRTEM图像显示AgNPs接近球形,平均尺寸为31.5 nm。从DLS研究中可以明显看出AgNPs的高负电位值(- 36.7 mV)。原子力显微镜(AFM)数据显示,颗粒呈球状凝聚,粒径小于40 nm。AgNPs对DPPH自由基的清除能力为66.72 %,具有良好的抗氧化活性。生物合成的AgNPs对爆发癌细胞表现出明显的抗肿瘤活性(MTT试验77.28 µg/mL),对革兰氏阴性菌和革兰氏阳性菌表现出良好的抑菌活性,抑菌区范围为8 ~ 16 mm。本研究展示了AgNPs的合成及其在生物医学上的应用前景,以及利用植物提取物制备绿色纳米颗粒的好处。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
0.40
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信