Genetic variation of white spot syndrome virus (WSSV) envelope protein VP28 influences its adaptivity in shrimps

IF 0.9 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-08-07 DOI:10.1016/j.genrep.2025.102310

SiouNing Aileen See , Subha Bhassu , Swee Seong Tang , Khatijah Yusoff

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引用次数: 0

Abstract

White spot syndrome virus (WSSV) is a significant viral pathogen in aquaculture leading to substantial economic loss. A comprehensive understanding of WSSV transmission, adaptation, and the mechanisms underlying shrimp survival and resistance is crucial. In this study, VP28 envelope protein gene from Malaysian WSSV isolates was sequenced and analysed using phylogenetic methods. The analysis revealed that VP28 gene shared over 87 % identity with other known WSSV isolates, as determined by NCBI BLAST. Phylogenetic analysis highlighted amino acid (aa) substitution at positions 42nd, 136th, and 180th to 183rd between Malaysia strains and those from other countries, potentially impacting the structure of VP28 and contributing to viral adaptability. Comparative studies of the VP28 gene's nucleotide composition and codon usage bias shed light on the molecular evolution of WSSV and its adaptation to hosts. Notably, comparisons between the VP28 sequences of Malaysian isolates from 2020 and 2022 revealed contrasting patterns: the 2020 strain exhibited higher nucleotide identity, whereas the 2022 strain had greater peptide identity compared to published VP28 sequences. These differences suggest that genetic variation and virulence of VP28 in global outbreaks may be influenced by host-pathogen interactions, environmental factors and selective pressures. This study offers insights into the evolutionary forces shaping the WSSV genome.

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白斑综合征病毒（WSSV）包膜蛋白VP28的遗传变异影响其在对虾中的适应性

白斑综合征病毒（White spot syndrome virus， WSSV）是水产养殖中一种重要的病毒性病原体，造成了巨大的经济损失。全面了解WSSV的传播、适应以及虾的生存和抗性机制至关重要。本研究对马来西亚WSSV分离株的VP28包膜蛋白基因进行了测序和系统发育分析。通过NCBI BLAST分析发现，VP28基因与其他已知WSSV分离株的同源性超过87%。系统发育分析显示，马来西亚病毒与其他国家病毒在42、136、180 ~ 183位点发生了氨基酸（aa）取代，这可能会影响VP28的结构，有助于病毒的适应性。VP28基因的核苷酸组成和密码子使用偏向性的比较研究有助于了解WSSV的分子进化及其对宿主的适应性。值得注意的是，2020年和2022年马来西亚分离株的VP28序列之间的比较揭示了截然不同的模式：与已发表的VP28序列相比，2020年菌株具有更高的核苷酸一致性，而2022年菌株具有更高的肽一致性。这些差异表明VP28在全球暴发中的遗传变异和毒力可能受到宿主-病原体相互作用、环境因素和选择压力的影响。这项研究提供了对形成WSSV基因组的进化力量的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.