A Chromosome-Level Genome Assembly and Annotation for the Beauty Snake Elaphe taeniura.

IF 2.5 2区生物学 Q2 EVOLUTIONARY BIOLOGY

Journal of Heredity Pub Date : 2025-08-16 DOI:10.1093/jhered/esaf055

Jiahao Chen, Qin Liu, Songwen Tan, Peng Guo, Lianming Du

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引用次数: 0

Abstract

The genus Elaphe Fitzinger, a large species clade within Colubridae, comprises 18 non-venomous snake species. Among them, Elaphe taeniura serves as a representative species due to its wide distribution and strong adaptability. However, genomic studies on this group remain limited. Here, we present a chromosome-level, high-quality reference genome for this species. The genome is assembled by integrating high-accuracy PacBio HiFi long-read sequencing and Hi-C chromatin conformation capture technologies and comprehensively annotated with the aid of transcriptomic data. The genome of E. taeniura spans 1.62 Gb, with a scaffold N50 of 206.4 Mb and the longest scaffold reaching 344.4 Mb. The genome exhibits a BUSCO completeness score of 98.2% and contains 22,246 protein-coding genes. Comparative analysis between the assembled genome and three other colubrid species revealed high synteny. In addition, several chromosomal fusion and fission events were observed. This reference genome provides a valuable resource for studying the taxonomy, conservation, and evolutionary history of the widely distributed Elaphe species.

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美蛇Elaphe taeniura染色体水平基因组组装与注释。

Elaphe Fitzinger属是蛇科中的一个大型物种分支，由18种无毒蛇组成。其中，Elaphe taeniura分布广泛，适应性强，是代表性物种。然而，对这一群体的基因组研究仍然有限。在这里，我们提出了一个染色体水平，高质量的参考基因组为该物种。基因组通过整合高精度PacBio HiFi长读测序和Hi-C染色质构象捕获技术进行组装，并借助转录组学数据进行全面注释。绦虫基因组全长1.62 Gb，支架N50为206.4 Mb，最长支架达到344.4 Mb， BUSCO完整性评分为98.2%，包含22,246个蛋白质编码基因。与其他三种杂交品种的基因组比较分析显示，其基因组具有较高的同源性。此外，还观察到一些染色体融合和裂变事件。该参考基因组为研究分布广泛的黄颡鱼的分类、保护和进化史提供了宝贵的资源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Heredity 生物-遗传学

CiteScore

5.20

自引率

6.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Over the last 100 years, the Journal of Heredity has established and maintained a tradition of scholarly excellence in the publication of genetics research. Virtually every major figure in the field has contributed to the journal. Established in 1903, Journal of Heredity covers organismal genetics across a wide range of disciplines and taxa. Articles include such rapidly advancing fields as conservation genetics of endangered species, population structure and phylogeography, molecular evolution and speciation, molecular genetics of disease resistance in plants and animals, genetic biodiversity and relevant computer programs.