Transcriptome analysis of genes and carbon partitioning pathways involved in high temperature stress resilience in groundnut (Arachis hypogaea L.).

IF 3.9 2区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
Rachana Bagudam, Anurag Mathew, Eswari Kancherla, Sai Rekha Kadirimangalam, Sudheer Kumar S, Prasad Bajaj, Narender Reddy S, Janila Pasupuleti
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Abstract

For the climate change scenarios where the temperatures during crop growing season are on the rise, tolerance to high-temperature (HT) stress in groundnut, especially during flowering and pod stages, is critical to sustain production in arid and semi-arid regions. To elucidate the regulatory mechanisms underlying the response of groundnuts to HT stress, a heat-tolerant (ICGV 16553) and a heat-sensitive (ICGV 16516) genotypes were identified based on responses in multi-season HT stress field experiments. The tolerant genotype had significantly higher total antioxidant activity and phenol content than the sensitive genotype in response to HT stress at flowering stage. Translocation studies revealed efficient sucrose loading into phloem, and increased glucose levels in the pods are indicative of an active sucrose metabolism in the tolerant genotype at the pod stage under HT stress. Transcriptomic analysis revealed a total of 1631 and 836 differentially expressed genes (DEGs) at flowering and pod stages, respectively. The identified DEGs encoded heat shock proteins, sucrose metabolism enzymes involved in photosynthate synthesis and translocation to sink tissues, transcriptional genes and antioxidants which played a major role in mitigating the effects of HT stress. The genes response in both sensitive and tolerant genotypes at each stage can provide valuable insights for future investigations and the genetic improvement of groundnut for heat tolerance mechanisms.

花生(arachhis hypogaea L.)高温胁迫恢复相关基因转录组分析及碳分配途径
在作物生长季节温度上升的气候变化情景下,花生对高温胁迫的耐受性,特别是在开花和结荚期,对维持干旱和半干旱地区的生产至关重要。为了阐明花生对高温胁迫响应的调控机制,通过多季高温胁迫试验,鉴定出了一个耐热基因型(ICGV 16553)和一个热敏基因型(ICGV 16516)。在花期对高温胁迫的响应中,抗性基因型的总抗氧化活性和酚含量显著高于敏感基因型。易位研究表明,蔗糖有效地装载到韧皮部,豆荚中葡萄糖水平的升高表明,在高温胁迫下,荚果期耐受基因型的蔗糖代谢活跃。转录组学分析显示,在开花和荚果期分别有1631个和836个差异表达基因(deg)。所鉴定的DEGs编码热休克蛋白、参与光合产物合成和转运到汇组织的蔗糖代谢酶、转录基因和抗氧化剂,在减轻高温胁迫的影响中起主要作用。敏感型和耐受性基因型在各阶段的基因响应可为花生耐热机制的进一步研究和遗传改良提供有价值的见解。
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来源期刊
Scientific Reports
Scientific Reports Natural Science Disciplines-
CiteScore
7.50
自引率
4.30%
发文量
19567
审稿时长
3.9 months
期刊介绍: We publish original research from all areas of the natural sciences, psychology, medicine and engineering. You can learn more about what we publish by browsing our specific scientific subject areas below or explore Scientific Reports by browsing all articles and collections. Scientific Reports has a 2-year impact factor: 4.380 (2021), and is the 6th most-cited journal in the world, with more than 540,000 citations in 2020 (Clarivate Analytics, 2021). •Engineering Engineering covers all aspects of engineering, technology, and applied science. It plays a crucial role in the development of technologies to address some of the world''s biggest challenges, helping to save lives and improve the way we live. •Physical sciences Physical sciences are those academic disciplines that aim to uncover the underlying laws of nature — often written in the language of mathematics. It is a collective term for areas of study including astronomy, chemistry, materials science and physics. •Earth and environmental sciences Earth and environmental sciences cover all aspects of Earth and planetary science and broadly encompass solid Earth processes, surface and atmospheric dynamics, Earth system history, climate and climate change, marine and freshwater systems, and ecology. It also considers the interactions between humans and these systems. •Biological sciences Biological sciences encompass all the divisions of natural sciences examining various aspects of vital processes. The concept includes anatomy, physiology, cell biology, biochemistry and biophysics, and covers all organisms from microorganisms, animals to plants. •Health sciences The health sciences study health, disease and healthcare. This field of study aims to develop knowledge, interventions and technology for use in healthcare to improve the treatment of patients.
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