Transcriptomic analysis reveals the impact of Rutin on the proliferation, barrier function, and transcription of Porcine IPEC-J2.

IF 2 3区农林科学 Q2 VETERINARY SCIENCES

Veterinary Research Communications Pub Date : 2025-08-15 DOI:10.1007/s11259-025-10858-x

Binbin Wang, Lele Huo, Wanru Tian, Wenwen Xing, Gang Luo, Mengxuan Li, Liumei Sun, Jiying Liu

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引用次数: 0

Abstract

The gastrointestinal health of livestock is essential for the agricultural sector. Rutin, a natural flavonoid extract from plants, exhibits antioxidant and anti-inflammatory properties, alongside a protective impact on the intestinal tract of pigs. However its influence on the proliferation, migration, and cellular junctions of IPEC-J2 cells remains unexplored. This study involved treating IPEC-J2 cells with varying concentrations of rutin. It was observed that after 24 h of treatment with 200 µM rutin, cell viability was significantly increased, along with significant upregulation in the mRNA expression levels of proliferation-related genes (CCNE2, CDK4, CDK2, CCNDB, and PCNA) and tight junction genes (ZO-1, Occludin, and Claudin-1). Additionally, the expression of proliferation-related PCNA protein was significantly increased. Transcriptome sequencing revealed that rutin treatment impacted the overall transcription profile of IPEC-J2 cells, altering the expression of genes involved in critical physiological processes such as redox balance, cell proliferation, and tight junctions maintenance. Consequently, rutin demonstrated a significant enhancement in the proliferation of IPEC-J2 cells and improved the intestinal barrier function in pigs, offering valuable insights for sustaining pig intestinal health in swine husbandry.

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转录组学分析揭示了芦丁对猪IPEC-J2增殖、屏障功能和转录的影响。

牲畜的肠胃健康对农业部门至关重要。芦丁是一种从植物中提取的天然类黄酮，具有抗氧化和抗炎特性，同时对猪的肠道有保护作用。然而，其对IPEC-J2细胞增殖、迁移和细胞连接的影响尚不清楚。本研究涉及用不同浓度的芦丁处理IPEC-J2细胞。结果发现，200µM芦丁处理24 h后，细胞活力显著提高，增殖相关基因（CCNE2、CDK4、CDK2、CCNDB、PCNA）和紧密连接基因（ZO-1、Occludin、Claudin-1） mRNA表达水平显著上调。增殖相关的PCNA蛋白表达显著升高。转录组测序显示，芦丁处理影响了IPEC-J2细胞的整体转录谱，改变了参与氧化还原平衡、细胞增殖和紧密连接维持等关键生理过程的基因的表达。因此，芦丁能显著促进猪IPEC-J2细胞的增殖，改善猪肠道屏障功能，为养猪业维持猪肠道健康提供有价值的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary Research Communications 农林科学-兽医学

CiteScore

2.50

自引率

0.00%

发文量

173

审稿时长

3 months

期刊介绍： Veterinary Research Communications publishes fully refereed research articles and topical reviews on all aspects of the veterinary sciences. Interdisciplinary articles are particularly encouraged, as are well argued reviews, even if they are somewhat controversial. The journal is an appropriate medium in which to publish new methods, newly described diseases and new pathological findings, as these are applied to animals. The material should be of international rather than local interest. As it deliberately seeks a wide coverage, Veterinary Research Communications provides its readers with a means of keeping abreast of current developments in the entire field of veterinary science.