Seed Transmission of Apple Stem Grooving Virus and Citrus Concave Gum-Associated Virus in Apple (Malus × domestica).

IF 3.1 2区 农林科学 Q2 PLANT SCIENCES
Anna O Wunsch, Mario Miranda Sazo, Janet van Zoeren, Kurt H Lamour, Oscar P Hurtado-Gonzalez, Joseph A Foster, Marc Fuchs
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引用次数: 0

Abstract

Viruses of cultivated apple (Malus × domestica) are believed to be transmitted nearly exclusively via vegetative propagation, with few known biotic vectors and limited evidence of vertical transmission. To evaluate the seed transmission capabilities of six viruses and one viroid of apple, a large-scale seedling grow-out experiment was conducted using seeds harvested from 51 trees infected by several combinations of six viruses and one viroid. Virus detection via multiplex PCR-based amplicon sequencing followed by RT-qPCR validation demonstrated that citrus concave gum-associated virus and apple stem grooving virus were transmitted to seedlings at rates of 4.0% (32/792) and 0.3% (3/908), respectively. No evidence of seed transmission was obtained for apple chlorotic leaf spot virus, apple green crinkle-associated virus, apple hammerhead viroid, apple rubbery wood virus 2, or apple stem pitting virus. These findings document a previously unknown mode of transmission for two widely distributed apple viruses with direct implications for breeding programs, the selection of virus-free trees, and the exchange of germplasm.

苹果茎沟病毒和柑橘凹胶相关病毒在苹果中的种子传播
栽培苹果(Malus × domestica)的病毒被认为几乎完全通过无性繁殖传播,很少有已知的生物媒介和有限的垂直传播证据。为评价苹果6种病毒和1种类病毒的传种能力,采用6种病毒和1种类病毒组合侵染的51棵果树种子,进行了大规模的出苗试验。通过多重pcr扩增子测序和RT-qPCR验证,柑橘凹胶相关病毒和苹果茎沟病毒的传毒率分别为4.0%(32/792)和0.3%(3/908)。苹果萎缩性叶斑病病毒、苹果绿皱相关病毒、苹果锤头类病毒、苹果橡胶木病毒2号和苹果茎麻蚀病毒没有种子传播的证据。这些发现记录了两种广泛分布的苹果病毒的一种以前未知的传播模式,这对育种计划、无病毒树的选择和种质交换具有直接意义。
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来源期刊
Phytopathology
Phytopathology 生物-植物科学
CiteScore
5.90
自引率
9.40%
发文量
505
审稿时长
4-8 weeks
期刊介绍: Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.
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