Investigation of the Efficacy of Umbilical Cord Mesenchymal Stem Cell and Melatonin Treatment in Premature Ovarian Failure Model.

IF 2.5 3区 医学 Q2 OBSTETRICS & GYNECOLOGY
Reproductive Sciences Pub Date : 2025-09-01 Epub Date: 2025-08-14 DOI:10.1007/s43032-025-01942-3
Aytaj Jafarzade, Elvan Anadol, Muzaffer Çaydere, Durmuş Burgucu, Canan Yılmaz, Semih Ergişi, Tamer Mungan
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引用次数: 0

Abstract

Premature Ovarian Insufficiency (POI) may develop in young female patients due to chemotherapy treatments, which causes infertility. In addition to stem cell treatment, which is an effective method for POI in recent years, melatonin, which has high antioxidant properties, is also known to be effective. This study aimed to investigate the efficacy of melatonin, human umbilical cord-derived mesenchymal stem cell (hucMSC), and melatonin + human umbilical cord-derived mesenchymal stem cell (hucMSC) treatments on POI in cyclophosphamide-induced POI models in rats. Sixty female animals were included in the experiment and randomly divided into the control, cyclophosphamide, cyclophosphamide + melatonin, cyclophosphamide + hucMSC, and cyclophosphamide + hucMSC + melatonin groups. Except for the control group, the POI model was created by administering intraperitoneal cyclophosphamide to other groups. Afterward, half of the animals were euthanized to investigate the effectiveness of the treatment, and AMH, E2, FSH, LH values in the blood of the euthanized animals were investigated by ELISA method. Follicle count was conducted in ovarian tissues, and the rate of apoptosis was determined by caspase-3 immunostaining. Fertility test was conducted on the group without euthanasia, and the number of baby rats was compared. Post-treatment E2 and AMH values were at the highest level in the cyclophosphamide + hucMSC + melatonin group. FSH value was at the lowest level in the cyclophosphamide + hucMSC + melatonin group. No significant difference was found between the groups in terms of LH value. Recovery was achieved with treatment in all follicle development stages. Particularly, the number of secondary and Graff follicles was statistically significantly higher in the cyclophosphamide + hucMSC + melatonin group. In caspase-3 immunostaining, the highest level of apoptosis was observed in the cyclophosphamide group, while the highest level of recovery was seen in the cyclophosphamide + hucMSC + melatonin group. As a result of fertility, the highest number of babies was observed in the cyclophosphamide + hucMSC + melatonin group following POI treatment. This study revealed that the success rate of POI treatment increases not only with stem cell treatment but also with combined treatment. If stem cell treatments are combined with melatonin with strong antioxidant properties, not as mono-therapy, for the purpose of infertility treatment, the effectiveness of the treatment increases.

脐带间充质干细胞联合褪黑素治疗卵巢早衰模型的疗效研究。
卵巢早衰(POI)可能发生在年轻女性患者由于化疗,导致不孕。除了干细胞治疗是近年来治疗POI的有效方法外,具有高抗氧化性能的褪黑素也被认为是有效的。本研究旨在探讨褪黑素、人脐带间充质干细胞(hucMSC)以及褪黑素+人脐带间充质干细胞(hucMSC)对环磷酰胺诱导的POI模型大鼠POI的影响。选取60只雌性动物,随机分为对照组、环磷酰胺组、环磷酰胺+褪黑素组、环磷酰胺+ hucMSC组和环磷酰胺+ hucMSC +褪黑素组。除对照组外,其余各组均腹腔注射环磷酰胺建立POI模型。之后,对一半的动物实施安乐死,观察治疗效果,并采用ELISA法检测安乐死动物血液中AMH、E2、FSH、LH值。卵巢组织进行卵泡计数,caspase-3免疫染色检测细胞凋亡率。对未安乐死组进行生育能力测试,比较幼鼠数量。治疗后E2和AMH值在环磷酰胺+ hucMSC +褪黑素组最高。环磷酰胺+ hucMSC +褪黑素组FSH值最低。两组间LH值无明显差异。在所有卵泡发育阶段治疗均可恢复。特别是,在环磷酰胺+ hucMSC +褪黑素组中,次级和格拉夫卵泡的数量有统计学意义上的增加。caspase-3免疫染色显示,环磷酰胺组细胞凋亡水平最高,而环磷酰胺+ hucMSC +褪黑素组细胞恢复水平最高。由于生育能力,POI治疗后环磷酰胺+ hucMSC +褪黑素组的婴儿数量最多。本研究表明,POI治疗的成功率不仅与干细胞治疗有关,而且与联合治疗有关。如果干细胞治疗与具有强抗氧化特性的褪黑激素联合使用,而不是作为单一疗法,以治疗不孕症,治疗的有效性会增加。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Reproductive Sciences
Reproductive Sciences 医学-妇产科学
CiteScore
5.50
自引率
3.40%
发文量
322
审稿时长
4-8 weeks
期刊介绍: Reproductive Sciences (RS) is a peer-reviewed, monthly journal publishing original research and reviews in obstetrics and gynecology. RS is multi-disciplinary and includes research in basic reproductive biology and medicine, maternal-fetal medicine, obstetrics, gynecology, reproductive endocrinology, urogynecology, fertility/infertility, embryology, gynecologic/reproductive oncology, developmental biology, stem cell research, molecular/cellular biology and other related fields.
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