Antimicrobiota vaccine induces lysine-mediated modulation of tick immunity affecting Borrelia colonization.

IF 3.2 3区生物学 Q2 MICROBIOLOGY

FEMS microbiology ecology Pub Date : 2025-08-23 DOI:10.1093/femsec/fiaf082

Lourdes Mateos-Hernandez, Lianet Abuin-Denis, Alejandra Wu-Chuang, Apolline Maitre, Helena Roháčková, Ryan O M Rego, Elianne Piloto-Sardiñas, James Valdes, Stefania Porcelli, Aurelie Heckmann, Sara Moutailler, Covadonga Lucas-Torres, Martin Moos, Stanislav Opekar, Myriam Kratou, Dasiel Obregon, Alejandro Cabezas-Cruz

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Abstract

Tick microbiota influences Borrelia colonization, but changes in the microbiota-derived metabolite and how this affects tick physiology and vector competence is unclear. We investigated whether microbiota-induced metabolite modifications influence tick physiology and pathogen transmission. Using an antimicrobiota vaccine (live Escherichia coli) to immunize mice, we generated host antibodies that modulated the tick microbiome, decreasing bacterial abundance and increasing lysine levels in ticks. Elevated lysine correlated with increased tick weight. Lysine supplementation experiments enhanced defensin expression with DefMT6 exhibiting anti-Borrelia activity, reducing pathogen load in ticks. Our findings demonstrate that antimicrobiota vaccines induce metabolite changes, affecting tick physiology, immunity, and vector competence. These insights open new avenues for developing microbiota-targeted strategies to control tick-borne diseases.

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抗微生物疫苗诱导赖氨酸介导的影响伯氏疏螺旋体定植的蜱免疫调节。

蜱虫微生物群影响伯氏疏螺旋体定植，但微生物群衍生代谢物的变化及其如何影响蜱虫生理和媒介能力尚不清楚。我们研究了微生物群诱导的代谢物改变是否会影响蜱虫生理和病原体传播。使用抗微生物疫苗（活大肠杆菌）对小鼠进行免疫，我们产生了调节蜱微生物组的宿主抗体，减少了蜱中的细菌丰度，增加了赖氨酸水平。赖氨酸升高与蜱虫体重增加相关。赖氨酸补充实验增强了防御素的表达，DefMT6具有抗疏螺旋体活性，减少了蜱的病原体负荷。我们的研究结果表明，抗微生物疫苗可诱导代谢物变化，影响蜱虫生理、免疫和媒介能力。这些见解为开发以微生物群为目标的策略来控制蜱传疾病开辟了新的途径。

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来源期刊

FEMS microbiology ecology 生物-微生物学

CiteScore

7.50

自引率

2.40%

发文量

132

审稿时长

3 months

期刊介绍： FEMS Microbiology Ecology aims to ensure efficient publication of high-quality papers that are original and provide a significant contribution to the understanding of microbial ecology. The journal contains Research Articles and MiniReviews on fundamental aspects of the ecology of microorganisms in natural soil, aquatic and atmospheric habitats, including extreme environments, and in artificial or managed environments. Research papers on pure cultures and in the areas of plant pathology and medical, food or veterinary microbiology will be published where they provide valuable generic information on microbial ecology. Papers can deal with culturable and non-culturable forms of any type of microorganism: bacteria, archaea, filamentous fungi, yeasts, protozoa, cyanobacteria, algae or viruses. In addition, the journal will publish Perspectives, Current Opinion and Controversy Articles, Commentaries and Letters to the Editor on topical issues in microbial ecology. - Application of ecological theory to microbial ecology - Interactions and signalling between microorganisms and with plants and animals - Interactions between microorganisms and their physicochemical enviornment - Microbial aspects of biogeochemical cycles and processes - Microbial community ecology - Phylogenetic and functional diversity of microbial communities - Evolutionary biology of microorganisms