Detection and Genetic Characteristics of Cosavirus and Salivirus in the Wastewater, China

IF 2.7 2区农林科学 Q2 ENVIRONMENTAL SCIENCES

Food and Environmental Virology Pub Date : 2025-08-15 DOI:10.1007/s12560-025-09659-x

Nan Zhou, Yue Huang, Tong Shen, Tingting Xiao, Hanyue Pang, Xinyu Shen

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引用次数: 0

Abstract

Cosavirus (CoSV) and Salivirus (SalV) are two novel picornaviruses that are associated with human diseases. However, epidemiological data on these viruses in China remain limited. In this study, we conducted the first simultaneous investigation of CoSV and SalV in a city of eastern China by wastewater-based epidemiology (WBE) method. From 2021 to 2023, a total of 21 influent wastewater samples were collected. After virus concentration and molecular detection, 71.43% (15/21) and 80.95% (17/21) of samples tested positive for CoSV and SalV, respectively. The sequences of CoSV were determined to be CoSV-A and CoSV-D, and SalV were all genotyped to be SalV-A1. Phylogenetic analysis showed that UTR region of CoSV-D and 3D region of CoSV-A can be divided into several clusters. UTR region of CoSV-A, UTR and 3D region of SalV-A1 were not clustered. Furthermore, the sequences in this study were closely related to some strains from human samples in China and wastewater samples in other countries. These findings reveal that CoSV and SalV circulated at a high prevalence in the local population and demonstrate that WBE is an effective method for novel picornaviruses research.

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中国污水中喉病毒和唾液病毒的检测及遗传特征

cosavvirus （CoSV）和Salivirus （SalV）是两种与人类疾病相关的新型小核糖核酸病毒。然而，中国关于这些病毒的流行病学数据仍然有限。本研究首次采用基于废水的流行病学（WBE）方法对中国东部城市的CoSV和SalV进行了同时调查。从2021年到2023年，共收集了21份进水废水样本。经病毒浓缩和分子检测，cosv15 /21、SalV阳性分别为71.43%（15/21）和80.95%（17/21）。经鉴定，CoSV基因序列为CoSV- a和CoSV- d， SalV基因型均为sav - a1。系统发育分析表明，CoSV-D的UTR区和CoSV-A的3D区可划分为多个聚类。CoSV-A的UTR区、sal - a1的UTR区和3D区未聚集。此外，本研究的序列与中国人类样本和其他国家废水样本中的一些菌株密切相关。这些结果表明，CoSV和SalV在当地人群中流行率很高，表明WBE是研究新型小核糖核酸病毒的有效方法。

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来源期刊

Food and Environmental Virology ENVIRONMENTAL SCIENCES-MICROBIOLOGY

CiteScore

6.50

自引率

2.90%

发文量

审稿时长

1 months

期刊介绍： Food and Environmental Virology publishes original articles, notes and review articles on any aspect relating to the transmission of pathogenic viruses via the environment (water, air, soil etc.) and foods. This includes epidemiological studies, identification of novel or emerging pathogens, methods of analysis or characterisation, studies on survival and elimination, and development of procedural controls for industrial processes, e.g. HACCP plans. The journal will cover all aspects of this important area, and encompass studies on any human, animal, and plant pathogenic virus which is capable of transmission via the environment or food.