Ruijie Hou, Jun Ye, Yang Zhang, Feng Hua, Bin Xu, Xuefeng Liu, Muhammad Qasim Mehmood, Jichuan Xiong
{"title":"Coherent-Excitation PACT With Frequency-Compensated Reconstruction for High-Contrast Deep-Tissue Imaging.","authors":"Ruijie Hou, Jun Ye, Yang Zhang, Feng Hua, Bin Xu, Xuefeng Liu, Muhammad Qasim Mehmood, Jichuan Xiong","doi":"10.1002/jbio.202500175","DOIUrl":null,"url":null,"abstract":"<p><p>Photoacoustic computed tomography (PACT) synergizes optical absorption contrast with ultrasonic resolution for noninvasive biomedical imaging yet faces limitations in signal-to-noise ratio (SNR), resolution, and contrast. This study introduces a coherent-excitation PACT system integrating interferometric optical excitation and Frequency-Compensated Filtered Back Projection (FC-FBP) reconstruction. The proposed method utilizes phase-locked dual-pulse interferometric excitation to amplify photoacoustic emissions; for the isolated chicken heart, the resolution is improved by 7.9% compared to the single-pulse protocol. The FC-FBP algorithm compensates for frequency-dependent acoustic attenuation via depth-adaptive Gaussian filtering, enhancing the projected signal in the target area while suppressing speckle artifacts. Through experimental validation, we confirm that the coherent-excitation scheme enables simultaneous optimization of optical fluence distribution and acoustic coherence; hence, it can be used to resolve previously indistinguishable hemoglobin oxygenation gradients in murine tumor models. This advancement establishes a high-sensitivity PACT framework, showing potential for real-time intraoperative imaging and dynamic metabolic monitoring in clinical applications.</p>","PeriodicalId":94068,"journal":{"name":"Journal of biophotonics","volume":" ","pages":"e202500175"},"PeriodicalIF":2.3000,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of biophotonics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/jbio.202500175","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Photoacoustic computed tomography (PACT) synergizes optical absorption contrast with ultrasonic resolution for noninvasive biomedical imaging yet faces limitations in signal-to-noise ratio (SNR), resolution, and contrast. This study introduces a coherent-excitation PACT system integrating interferometric optical excitation and Frequency-Compensated Filtered Back Projection (FC-FBP) reconstruction. The proposed method utilizes phase-locked dual-pulse interferometric excitation to amplify photoacoustic emissions; for the isolated chicken heart, the resolution is improved by 7.9% compared to the single-pulse protocol. The FC-FBP algorithm compensates for frequency-dependent acoustic attenuation via depth-adaptive Gaussian filtering, enhancing the projected signal in the target area while suppressing speckle artifacts. Through experimental validation, we confirm that the coherent-excitation scheme enables simultaneous optimization of optical fluence distribution and acoustic coherence; hence, it can be used to resolve previously indistinguishable hemoglobin oxygenation gradients in murine tumor models. This advancement establishes a high-sensitivity PACT framework, showing potential for real-time intraoperative imaging and dynamic metabolic monitoring in clinical applications.