Decoration of gold nanoparticles with glycopeptides leads to a lower cellular uptake and liver retention†

IF 4.6 3区 材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY
Mahmoud G. Soliman, Jennifer Fernandez Alarcon, Tanja Ursula Lüdtke, Martina B. Violatto, Marko Dobricic, Chiara Cordiglieri, Alessandro Corbelli, Fabio Fiordaliso, Giovanni Sitia, James S. O'Donnell, Daniel I. R. Spencer, Sergio Moya, Paolo Bigini and Marco P. Monopoli
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Abstract

Unspecific uptake by the liver is one of the main drawbacks of the translation of nanomaterials into clinics, preventing their delivery into diseased tissues. Here, we synthesized gold nanoparticles (GNPs) decorated with a sialic acid-displaying glycopeptide to enhance their specific targeting properties by reducing their uptake inside hepatic cells. We demonstrated the biocompatibility of the glycopeptide-coated GNPs with two different nanomaterial shapes (spherical and rod-like GNPs) and the targeting properties of the glycopeptide were retained in serum-free and protein-rich media. We found that the glycopeptide reduces nanomaterial interaction with hepatic cells by 1.96 times. In the liver, Kupffer cells (KCs) and liver sinusoidal endothelial cells (LSECs) were the only cells that interacted with the GNPs, increasing the expression of sialic acid-binding receptors such as Siglec-1. This work provides potential new strategies to overcome off-target nanomaterial accumulation by manipulating nanomaterial functionalisation with glycans to alter hepatic cell interactions.

Abstract Image

用糖肽修饰金纳米颗粒可降低细胞摄取和肝脏滞留。
肝脏的非特异性摄取是将纳米材料转化为临床的主要缺点之一,这阻碍了它们进入病变组织。在这里,我们合成了用唾液酸显示糖肽修饰的金纳米颗粒(GNPs),通过减少其在肝细胞内的摄取来增强其特异性靶向特性。我们证明了糖肽包被的GNPs与两种不同纳米材料形状(球形和棒状GNPs)的生物相容性,并且糖肽的靶向特性在无血清和富含蛋白质的培养基中保持不变。我们发现糖肽使纳米材料与肝细胞的相互作用减少了1.96倍。在肝脏中,库普弗细胞(KCs)和肝正弦内皮细胞(LSECs)是唯一与GNPs相互作用的细胞,增加了唾液酸结合受体(如siglec1)的表达。这项工作提供了潜在的新策略,通过聚糖操纵纳米材料功能化来改变肝细胞的相互作用,从而克服脱靶纳米材料的积累。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Nanoscale Advances
Nanoscale Advances Multiple-
CiteScore
8.00
自引率
2.10%
发文量
461
审稿时长
9 weeks
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