The horse gut bacteriome and anaerobic mycobiome are influenced by seasonal forages and small intestinal starch digestibility.

IF 3.2 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of Applied Microbiology Pub Date : 2025-09-01 DOI:10.1093/jambio/lxaf203

Georgia Wunderlich, Michelle Bull, Nerida McGilchrist, Chenchen Zhao, Tom Ross, Michael Rose, Belinda Chapman

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引用次数: 0

Abstract

Aims: The equine gut microbiome is a complex and dynamic ecosystem, housing a diverse consortium of bacteria and anaerobic fungi (AF) capable of breaking down complex plant matter and converting it into vital energy sources for their host. The aim of this study was to broaden our current understanding of bacterial and AF diversity in the equine hindgut and how it differs between cohorts and responds to dietary shifts.

Methods and results: Faecal samples were collected from 48 horses and the bacteriome and anaerobic mycobiome analysed using long-read amplicon sequencing. Samples were collected from racehorses (RH) on high-concentrate diets and wild-foraging (WF) horses grazing on seasonally variable pastures. Distinct microbial profiles were observed, with bacterial diversity and composition strongly influenced by season. Reduced hindgut starch availability in RH promoted fibrolytic taxa such as Roseburia and reduced amylolytic Lactobacillus. AF communities, though relatively stable across dietary and seasonal changes, differed between the horse cohorts. Piromyces and Khoyollomyces were found to be more strongly associated with WF horses and Caecomyces with RH.

Conclusion: These findings emphasize the dynamic interplay between diet and microbial composition. Future multi-omics research will enhance our understanding of microbial functionality and inform strategies to optimize equine gut health.

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马肠道菌群和厌氧菌群受季节牧草和小肠淀粉消化率的影响。

目的：马肠道微生物群是一个复杂而动态的生态系统，容纳了多种细菌和厌氧真菌（AF），它们能够分解复杂的植物物质并将其转化为宿主的重要能量来源。本研究的目的是扩大我们目前对马后肠细菌和房颤多样性的理解，以及它在队列之间的差异和对饮食变化的反应。方法与结果：采集48匹马的粪便样本，采用长读扩增子测序对细菌组和厌氧真菌组进行分析。样品采集于饲喂高精料日粮的赛马（RH）和饲喂季节变化草场的野生觅食马（WF）。观察到不同的微生物分布，细菌多样性和组成受季节影响较大。RH后肠淀粉利用率的降低促进了纤维分解类群，如玫瑰菌，并减少了淀粉分解乳酸杆菌。尽管在饮食和季节变化中AF群落相对稳定，但在马群之间存在差异。piroomyces和khoyolomyces与WF马的相关性更强，caomomyces与RH的相关性更强。结论：这些发现强调了饮食与微生物组成之间的动态相互作用。未来的多组学研究将增强我们对微生物功能的理解，并为优化马肠道健康提供信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Microbiology 生物-生物工程与应用微生物

CiteScore

7.30

自引率

2.50%

发文量

427

审稿时长

2.7 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.