Optoretinography with actively stabilized adaptive optics optical coherence tomography.

IF 3.2 2区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Biomedical optics express Pub Date : 2025-07-17 eCollection Date: 2025-08-01 DOI:10.1364/BOE.566376

Jason H Wong, Shangbang Luo, Zohreh Hosseinaee, Fabio Feroldi, Austin Roorda

{"title":"Optoretinography with actively stabilized adaptive optics optical coherence tomography.","authors":"Jason H Wong, Shangbang Luo, Zohreh Hosseinaee, Fabio Feroldi, Austin Roorda","doi":"10.1364/BOE.566376","DOIUrl":null,"url":null,"abstract":"<p><p>Optoretinography (ORG) is the optical measurement of changes in the retina in response to light stimulation. Adaptive optics optical coherence tomography (AOOCT) records photoreceptor ORGs by measuring the physical changes in their outer segment lengths in response to light stimulation. The main difficulty in recording these nanometer-scale changes is constant eye motion. Typically, fast volume acquisitions are used with offline spatial registration to compensate for the effect of eye motion. Here, we present an alternate solution whereby an adaptive optics scanning light ophthalmoscope (AOSLO) is used to measure the eye motion and actively guide the AOOCT beam to compensate for eye motion in real time. This system's cellular-scale tracking offers unparalleled control over scanning raster size and shape, allowing for high-speed (up to 100 kHz) ORG acquisition from targeted locations. We validate the method by comparing cone classifications against those made with an established ORG approach.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 8","pages":"3222-3236"},"PeriodicalIF":3.2000,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12339298/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical optics express","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1364/BOE.566376","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/8/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

Abstract

Optoretinography (ORG) is the optical measurement of changes in the retina in response to light stimulation. Adaptive optics optical coherence tomography (AOOCT) records photoreceptor ORGs by measuring the physical changes in their outer segment lengths in response to light stimulation. The main difficulty in recording these nanometer-scale changes is constant eye motion. Typically, fast volume acquisitions are used with offline spatial registration to compensate for the effect of eye motion. Here, we present an alternate solution whereby an adaptive optics scanning light ophthalmoscope (AOSLO) is used to measure the eye motion and actively guide the AOOCT beam to compensate for eye motion in real time. This system's cellular-scale tracking offers unparalleled control over scanning raster size and shape, allowing for high-speed (up to 100 kHz) ORG acquisition from targeted locations. We validate the method by comparing cone classifications against those made with an established ORG approach.

查看原文本刊更多论文

主动稳定自适应光学光学相干层析成像。

视网膜造影（ORG）是对视网膜响应光刺激变化的光学测量。自适应光学光学相干断层扫描（AOOCT）通过测量光感受器外节长度在光刺激下的物理变化来记录光感受器ORGs。记录这些纳米级变化的主要困难是持续的眼球运动。通常，快速体积获取与离线空间配准一起使用，以补偿眼球运动的影响。在这里，我们提出了一种替代解决方案，即使用自适应光学扫描光检眼镜（AOSLO）来测量眼球运动并主动引导AOOCT光束实时补偿眼球运动。该系统的蜂窝尺度跟踪提供了对扫描光栅尺寸和形状的无与伦比的控制，允许从目标位置进行高速（高达100 kHz）的ORG采集。我们通过将锥分类与已建立的ORG方法进行比较来验证该方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biomedical optics express BIOCHEMICAL RESEARCH METHODS-OPTICS

CiteScore

6.80

自引率

11.80%

发文量

633

审稿时长

1 months

期刊介绍： The journal''s scope encompasses fundamental research, technology development, biomedical studies and clinical applications. BOEx focuses on the leading edge topics in the field, including: Tissue optics and spectroscopy Novel microscopies Optical coherence tomography Diffuse and fluorescence tomography Photoacoustic and multimodal imaging Molecular imaging and therapies Nanophotonic biosensing Optical biophysics/photobiology Microfluidic optical devices Vision research.